A sensitive and rapid method was developed for the determination of cAMP in Locusta migratoria manilensis Meyen by high-performance liquid chromatography with fluorescence detection. The cAMP was derivatized using chloroacetaldehyde and TBAS buffer/methanol was used as the mobile phase. A detection quantification of 40 fmol/ml could be achieved when using fluorescence detection. An HPLC-MS meth...

The connective tissue sheath surrounding the nervous system of Locusta migratoria has been studied histochemically. It consists of an outer non-cellular layer, the neural lamella, and an inner layer of cells, the sheath-cells. The neural lamella has been identified as being composed of a collagen-type protein and neutral mucopolysaccharide on the evidence of its histochemical reactions and the ...

Wanderheuschrecken (Locusta migratoria) veratmen während eines längeren Fluges in den ersten dreißig Minuten vor allem Kohlenhydrate, später Fette1. In ihren Flugmuskeln können sowohl die Enzyme des Fettals auch die des Kohlenhydratabbaues nachgewiesen werden. Außerdem sinkt wäh­ rend des Fluges ihr Gehalt an Glykogen, Trehalose und Glukose2. Humorale, vielleicht auch nervöse Faktoren dürften d...

In the locust, Locusta migratoria, the activity of identified efferent neurones of the suboesophageal ganglion was recorded extracellularly for long periods (hours or days). During recording, the animals were free to move in their enclosures. Activity of the cells appears to accompany feeding behaviour: they become active shortly before feeding starts and their activity persists during feeding....

The unpaired X chromosome of Locusta migratoria males is facultatively heterochromatic in the spermatocytes and early spermatids, showing an alternating cycle of positive-negative-positive heteropycnosis. Discrimination between facultative heterochromatin and euchromatin was made using a modification of the Feulgen reaction. It was found that the differential stainability of the DNA of the X ch...

Microsporidia Paranosema locustae and Paranosema grylli infect fat bodies of orthopteran hosts Locusta migratoria and Gryllus bimaculatus, respectively, and cause formation of nodules consisting of deposits of melanin around heavily infected cells. Both species sporadically produce enlarged or malformed (teratoid) spores as a result of abnormal sporogony. Proportions of teratospores within mela...

Together with type A GABA and strychnine-sensitive glycine receptors, glutamate-gated chloride channels (GluCl) are members of the Cys-loop family of ionotropic receptors, which mediate fast inhibitory neurotransmission. To date, GluCls are found in invertebrates only and therefore represent potential specific targets for insecticides, such as ivermectin and fipronil. In this study, we identifi...

Flight muscle and fat body extracts from Locusta migratoria were incubated with D-[U-14C]-glucose or D-[3-3H]-3-deoxy-3-fluoroglucose and the products were analyzed. In the case of the latter compound, radio-chromatographic analysis yielded glycogen and trehalose fractions that were shown by 19F nuclear magnetic resonance to contain fluorine. Acid hydrolysis of these fractions liberated tritium...