abstract the ability to monitor particular microorganism in an environment is a difficult task. many genes permit the differentiation of strains by conferring production of unique phenotypes such as bioluminescence [marine bacterial luciferase (luxab)]. therefore, lux-ab gene was cloned into two endemic plant pathogens pseudomonas syringae and ralostonia solanacearum by electrotransformation. l...

The high-luminosity upgrade of the LHC (HL-LHC) is foreseen to reach an instantaneous luminosity a factor five seven times nominal design value. This creates need for new high-precision instrumentation at CMS, using radiation-hard detector technologies. contribution introduces bunch-by-bunch online and beam-induced background measurements in CMS. Tracker Endcap Pixel Detector (TEPX) will be ada...

Ascorbic acid (AA) has antioxidant properties. However, in the presence of Fe2+/Fe3+ ions and H2O2, it may behave as a pro-oxidant by accelerating enhancing formation hydroxyl radicals (•OH). Therefore, this study we evaluated effect AA at concentrations 1 to 200 µmol/L on •OH-induced light emission (at pH 7.4 temperature 37 °C) from 92.6 Fe2+—185.2 EGTA (ethylene glycol-bis (β-aminoethyl ether...

Benzalkonium chloride (BAC) is a chlorine-based chemical compound with proven antimicrobial properties against bacteria, viruses, and fungi, depending on the length of alkyl chain. It can be used as biocide, cationic surfactant, phase transfer agent. The aim this study was to investigate effectiveness commercial cleaning agents for sanitary areas Bis duo Active (BDA) BAC an active in working co...

Coastal region of Chah Bahar port, Sea of Oman, was screened for the presence of bioluminescence bacteria for the first time. Water samples were taken from surface and subsurface layers and immediately spread on nutrient seawater complete (SWC) agar. Luminous colonies were observed after an overnight incubation at 25°C. Among twenty luminous isolates, four of them were selected for preliminary ...

The life cycle of Trypanosoma brucei brucei involves a series of differentiation steps characterized by marked changes in mitochondrial development and function. The bloodstream forms of this parasite completely lack cytochromes and have not been considered to have any Krebs cycle function. It has been suggested that glycolysis is the sole source of ATP in all bloodstream forms. However, earlie...

The sensitivity, accuracy, and precision of the CU compared favorably with those of the CYCLO-Trac SP RIA. Both procedures take about the same time to perform. The effort involved in the preparation of the CU reagents is slight and can be accomplished by any competent technologist. The magnetic solid-phase separation and wash steps are rapidly performed. The automatic dispensing and mixing of r...

OBJECTIVES To determine the mechanism of antimicrobial action of lactocin 160, a bacteriocin produced by the healthy vaginal strain of Lactobacillus rhamnosus, using an established model, with Micrococcus luteus ATCC 10420 as a test organism. METHODS Sensitivity of M. luteus to lactocin 160 was determined by the diffusion assay. Loss of cellular ATP in the lactocin-treated cells was elucidate...

Protein-protein interactions (PPIs) constitute the regulatory network that coordinates diverse cellular functions. There are growing needs in plant research for creating protein interaction maps behind complex cellular processes and at a systems biology level. However, only a few approaches have been successfully used for large-scale surveys of PPIs in plants, each having advantages and disadva...

This study investigated the correlation between sperm motion parameters obtained by a computer-assisted semen analyzer and levels of reactive oxygen species in unwashed semen. In total, 847 patients, except for azoospermic patients were investigated. At the time of each patient's first consultation, semen parameters were measured using SMAS™ or CellSoft 3000™, and production of reactive oxygen ...