The diagnosis of hairy cell leukemia was made in three patients by phase-contrast microscopy and histochemistry of the abnormal peripheral blood cells. Both IgM and IgD surface immunoglobulins were resynthesized after these cells were trypsinized and cultured. Aggregate or Fc receptors were demonstrated on hairy cells. The ability to phagocytose latex was also a property of hairy cells; however...

When human lymphocytes are cultured in the presence of phytomitogens, 70-90% of the cells undergo blast transformation and synthesize DNA. However, less than 40% of these lymphocytes actually undergo mitosis while 35-90% of the newly synthesized DNA is excreted into the media. The release of DNA by the cells is selective since experiments with [(14)C]uridine indicate that RNA is not lost into t...

A study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA)-induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 dif...

By CHARLES N. GAMBLE O VER THE PAST 5 YEARS considerable interest has been shown in the plant lcctin phytohcmagglutinin ( PHA ) , ol)tained from extracts of the red kidney bean Phaseolus vulgaris. This Iectin is a potent erythroagglutinin which is also capable of agglutinating leukocytes and apparently stimulating the majority of small lymphocytes in human peripheral blood cultures to transform...

The biological effect of visible light of low energy density was investigated in this study. The effects of diffuse (DL) and linearly polarized (LPL) light were compared on models in vitro and in vivo. Experiments in vitro were performed on human lymphocytes to study their blast-transformation and rosette-formation abilities. Both DL and LPL increased the number of blast-transformed cells even ...

I TARIOUS CLINICAL AND EXPERIMENTAL STUDIES have shown V that chioramphenicol ( CAP) affects cell division. #{176} The characteristic aplastic marrow that is seen when toxicity is fully expressed suggests a toxic effect leading to depletion of dividing cells. The effect of CAP on incorporation of 32 into phytohemagglutinin ( PHA) stimulated lymphocytes was reported by McIntyre and Ebaugh,’ who ...

Transformation of chronic lymphocytic leukemia to acute lymphoblastic leukemia is extremely rare. We describe an elderly patient whose lymphocytes on presentation were morphologically characteristic of chronic lymphocytic leukemia, but changed to lymphoblasts in a very short interval. Peripheral blood mononuclear cells were tested for surface immunoglobulins and T65 antigen at initial presentat...

ABSTRACT. EBV infection is one of the most common infections in world. About 90 % world's population infected with Epstein-Barr virus. The virus capable affecting immunocompetent cells, causing lymphadenopathy, hepato- and splenomegaly, being stored forever body's lymphocytes, an immunodeficient state. aim study. purpose study was to determine relationships between nature course disease for inf...

The animals used were inbred Hartley strain guinea pigs (400-500 g), inbred Sprague strain rats (300400 g), and a purebred Dutch rabbit (1,300 g). Lymphocytes were obtained from the guinea pig and rat lymph nodes and the rabbit spleen. The animals were anesthetized with ethyl ether and exsanguinated via caridac puncture. The blood was defibrinated, and the serum was collected and filtered throu...