In this paper we show that hydroxyapatite nanoparticles (NP) can not only act as a chaperon (by imparting thermostability) but can serve as a synthetic enhancer of activity of an isolated extracellular pectate lyase (APL) with low native state activity. The purified enzyme (an attenuated strain of Macrophomina phaseolina) showed feeble activity at 50°C and pH 5.6. However, on addition of 10.5 µ...

Macrophomina phaseolina é um fitopatógeno de solo com ampla distribuição geográfica, responsável por ocasionar perdas econômicas graves incitando sintomas podridões no sistema radicular e caule das plantas infectadas. Há uma demanda mundial crescente novos tratamentos, que promovam a segurança alimentar nutricional, substâncias mais eficazes menos danosas ao equilíbrio dos ecossistemas. O objet...

abstract temperature is one of the most important environmental factors affecting on physiological characteristics of fungi. in order to wide host range of macrophomina phaseolina (tassi) goildanich, verticillium dahliae kleb. and sclerotinia sclerotiorum (lib.) de bary, become aware from growth rate and productivity of resistant and survival form of these phyto-pathogens in different temperatu...