We describe a novel fluorometric technique for simple, rapid, and economical assay of glucose by use of immobilized glucose oxidase and peroxidase. A cylindrical magnetic stirrer was specially designed to hold the immobilized enzymes firmly and to allow the reaction minzymic transformation quickly. Blood plasma can be assayed directly with no pretreatment. Ascrobic acid, uric acid, and creatini...

Nanotechnology has become an important and interesting field of physics, chemistry, biology engineering in recent years. The rapidly growing nanotechnology today is nanoparticles. characteristic nanoparticles that the particle size very small range (1-100 nm). Silver have been synthesized using saga leaf extract (Abrus pecatorius L.) as a reducing agent. formation silver was carried out by addi...

C. CRITICAL CONTROL POINTS AND MINIMUM STANDARDS 2 Figure 1: Example of a magnetic stirrer method for pooled sample digestion (steps labelled as 1-4 indicate the required sequential order for preparing the digest). 3 C1 Sample collection and preparation for testing 4 C2 Minimum requirements for equipment and consumables 5 C3 Performance of the digestion assay 6 C3.1 Blending of muscle samples 6...

Cleaning the Apparatus After each series of examinations the reaction chamber is cleaned with a 1%0 solution of triethanolamine stained with phenol red; this is followed by lactic acid water. When the mercury becomes dirty and begins to form little balls in the reaction chamber I to 2 ml. of 200,, ferricyanide solution is introduced into the reaction chamber and shaken well. Lactic acid and wat...

We describe a semiautomated batch-assay system designed to increase the speed and ease of performing radioimmunoassays while maintaining good accuracy and precision. The main components are a programmable pipetting unit (sample processor) and a gamma counter capable of simultaneously counting radioactivity in 12 samples. The processor uses prearranged sets of as many as 24 disposable pipette ti...

Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conduc...