Microchannel emulsification can produce droplets of uniform size at high throughput for wide-ranging applications in the food and drug industry. Mammalian cell encapsulation using emulsion-based systems is significant interest immobilized culture or transplantation applications. has recently been applied to mammalian alginate. Here, we extend this approach thermoresponsive hydrogels chitosan as...

Polymeric Nanoneedle Arrays In article number 2104828, Yaping Chen, Nicolas H. Voelcker, Roey Elnathan, and co-workers demonstrate the fabrication of relatively low-cost high throughput polymeric nanoneedles from cell culture polystyrene. The with precise geometry are imprinted directly on polystyrene petri dish via nanoimprint lithography. arrays can precisely manipulate cellular processes med...

objective: to study the mechanisms involved in amyloid formation processes, we made a mammalian cell culture model of amylin aggregation and characterized its properties. materials and methods: amyloid fibrils were extracted from cho cells and binding affinity to thioflavin t and congo red was investigated. then the apple-green birefringence of extracted fibrils was detected under polarized li...

UNLABELLED Cultivation of primary cells is essential for biotechnological research and viral vaccine production. Significant advances in cell and tissue culture, more specifically, advances in the transfection and transduction of human and mammalian cells, has directly led to giant leaps forward in fields such as cancer research, genetics, and public health. At the same time, a corresponding in...

Culture medium used in antibody purification contains growth factors, but is usually discarded after antibody purification. We examined to determine whether the growth factors can be (i) recovered and (ii) used in subsequent cell culture. Using this medium enhanced the survival of hybridoma cells and improved antibody production. Culture medium previously used in antibody purification offers an...

We have examined the specificity of communication between cells in culture by co-culturing cells derived from mammalian, avian, and arthropod organisms. Both mammalian and avian culture cells have similar gap junctional phenotypes, while the insect (arthropod) cell lines have a significantly different gap junctional structure. Electrophysiological and ultrastructural methods were used to examin...

conclusions our underlying hypothesis is that recapitulating the three-dimensional early embryonic structure, in order to reproduce better in vitro the three-dimensional morphogenetic-like re-arrangements, would improve cells differentiation, when in vivo transplanted; moreover, it could be used as a simplified cancer disease model and reliable drug evaluation method as well. results the proces...