BACKGROUND This study is performed to reveal most common species and subspecies of leptospires that are main causes of human leptospirosis in Guilan, Northern Province of Iran. METHODS We performed IgM-ELISA and MAT on 282 blood samples from patients who attended to 3 hospitals in the flat area of Guilan Province with clinical symptoms consisted with leptospirosis. All specimens with titers ≥...

  Leptospirosis is an acute infectious, systemic and septisemic disease which had recent outbreaks in some parts of Iran especially in north provinces. Rapid detection is a critical step for treatment and control of this disease. In this research a PCR based method was evaluated for detection of Iranian local endemic serovars. All reported endemic serovars of Leptospira including Leptospira gri...

A total of 400 serum samples collected from patients, clinically suspected of leptospirosis, were evaluated for antibodies by LEPTO dipstick and microscopic agglutination test (MAT). Twenty of these patients (5%) had serological evidence of leptospirosis. Leptospira interrogans serovars Autumnalis and Icterohaemorrhagiae, Canicola and Javanica were serogroups recorded serologically. Fever and j...

BACKGROUND Leptospirosis is a potentially lethal zoonosis mainly affecting low-resource tropical countries, including Peru and its neighbouring countries. Timely diagnosis of leptospirosis is critical but may be challenging in the regions where it is most prevalent. The serodiagnostic gold standard microagglutination test (MAT) may be technically prohibitive. Our objective in this study was to ...

were anicteric leptospirosis.1 Main presentation in our cases was Weil’s disease, so, it is difficult to draw conclusions from only two patients of anicteric leptospirosis. As far as the study of Elizabeth F Daher et al is concerned this study also had Jaundice in 94.5% patients out of total 201 patients.2 MAT is a highly specific test, though sensitivity is poor, whereas mildly raised transami...

Leptospirosis is a widespread zoonotic disease that affects all mammals, including humans, in different parts of the world. Clinical recognition of leptospirosis is challenging, and the definitive serologic diagnosis assay, the microscopic agglutination test (MAT), is time-consuming and difficult to conduct. In this study, an indirect immunoperoxidase (IIP) test to detect Leptospira-specific an...

A rapid IgM-detection immunochromatographic test (WITNESS® Lepto, Zoetis) has recently become available to identify acute canine leptospirosis at the point of care. Diagnostic sensitivity and specificity of the test were evaluated by comparison with the microscopic agglutination assay (MAT), using a positive cut-off titer of ≥800. Banked serum samples from dogs exhibiting clinical signs and sus...

Early diagnosis based on laboratory confirmation is essential for managing leptospirosis. This study investigated the effectiveness of a novel method of detecting leptospirosis that combines measurement of anti-Leptospira antibodies by the microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA), and immunochromatographic test (ICT) and leptospiral DNA by loop-mediated is...

INTRODUCTION Assays based on DNA amplification can provide information that contributes to the initial management of patients with leptospirosis. However, these have not been adopted in Uruguay. Our aim was to evaluate the performance of the lipL32 real-time PCR (qPCR) for diagnosis of leptospirosis. METHODOLOGY We analyzed by microscopic agglutination test (MAT) and lipL32 qPCR serum samples...

Leptospirosis is an important emerging zoonotic infection in developing regions of the world. Laboratory testing for leptospiral infections is important both for diagnosis and management of the patients. The conventional laboratory methods for diagnosis have many shortcomings. The availability of molecular techniques like Polymerase Chain Reaction (PCR) as diagnostic tool has improved speed and...