UNLABELLED Absolute quantitation of myocardial blood flow (MBF) by PET is an established method of analyzing coronary artery disease (CAD) but subject to the various shortcomings of available radiotracers. Flurpiridaz F 18 is a novel PET radiotracer that exhibits properties of an ideal tracer. METHODS A new absolute perfusion quantitation method with flurpiridaz was developed, taking advantag...

In fission yeast cells, Cds1 is the effector kinase of the DNA replication checkpoint. We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1 is phosphorylated and inactivated by Cds1, resulting in activation of MluI-binding factor (MBF)-dependent transcription. This is essential to reinitiate DNA synthesis and for correct G1-to-S transition. Here we show...

Myocardial perfusion MRI is acquired with a T1-weighted dynamic MRI sequence. Fully quantitative analysis of myocardial perfusion MRI allows the absolute quantification of myocardial blood flow (MBF) through the use of complex mathematical modeling. There are essentially two main methods for quantification of absolute MBF: the linear time-invariant model and the compartment model. Maximized con...

We investigated the effect of insulin on total and regional myocardial blood flow (MBF) and glucose uptake (MGU) in healthy subjects (50 +/- 5 yr) by means of positron emission tomography (PET) with oxygen-15-labeled water (H(2)(15)O) and fluorine-18 labeled fluorodeoxyglucose ((18)FDG) before and during physiological hyperinsulinemia (40 mU.min(-1).m(-2)). Twelve male subjects were included in...

and Symptoms in Patients With Idiopathic Dilated Cardiomyopathy To the Editor: With great interest we read the article by Node et al1 concerning short-term statin therapy in patients with idiopathic dilated cardiomyopathy (IDC). The demonstrated improvement of left ventricular ejection fraction (LVEF) by simvastatin therapy is intriguing. The authors speculated on several possible mechanisms, i...

Changes in vascular conductance (VC) are required to counter changes in muscle perfusion pressure (MPP) to maintain muscle blood flow (MBF) during exercise. We investigated the recruitment of VC as a function of peak VC measured in three body positions at two different work rates to test the hypothesis that adaptations in VC compensated changes in MPP at low-power output (LPO), but not at high-...

BACKGROUND The aim of this study was to determine whether epicardial structural disease may affect the manifestation of a longitudinal decrease in myocardial blood flow (MBF) or MBF difference during hyperemia in cardiovascular risk individuals, and its dependency on the flow increase. METHODS AND RESULTS In 54 cardiovascular risk individuals (at risk) and in 26 healthy controls, MBF was meas...

OBJECTIVES The purpose of this study was to evaluate the diagnostic value of a positron emission tomography (PET)/computed tomography (CT)-determined longitudinal decrease in myocardial blood flow (MBF) gradient during hyperemia and myocardial flow reserve (MFR) for the identification of epicardial stenosis ≥50%. BACKGROUND Although PET-determined reductions in MFR are increasingly applied to...

PURPOSE To compare the dual-bolus to single-bolus quantitative first-pass magnetic resonance myocardial perfusion imaging for estimation of absolute myocardial blood flow (MBF). MATERIALS AND METHODS Dogs had local hyperemia of MBF in the left anterior descending (LAD) coronary artery (intracoronary adenosine). Animals (n = 6) had sequential single- and dual-bolus perfusion studies with micro...

Materials and Methods: Nine canine models of left anterior descending artery stenosis were prepared and underwent MDCT perfusion imaging during adenosine infusion to study a wide range of flow parameters. Neutron-activated microspheres were injected to document MBF during adenosine infusion. Six animals underwent dynamic MDCT perfusion imaging, and K1 and k2 (which represent the first-order tra...