Monoclonal antibodies directed against rabbit reticulocyte protein synthesis initiation factor 4A (eIF-4A) were used to isolate mouse cDNA clones expressing eIF-4A protein sequences in E. coli. The identity of cDNA clones encoding eIF-4A sequences was confirmed by hybrid-selected translation and peptide mapping of the translation product. Analysis of the mRNA coding for eIF-4A from mouse liver ...

Cyclization and chlorination of 2-amino-5-benzyl-4, 5, 6, 7-tetrahydro hydro thieno [3, 2c] pyridine-3carboxamide yielded (3 a-b). Reaction with different substituted pyridines of [3 a-b] yielded 4a (1-4) and 4b (1-2). However reaction of 3a with different substituted 2-amino phenols yielded 4a (5-6). Further 3a yields 4a (7) & 4a (8) from different substituents like o-phenylene diamine and 4-c...

To investigate the possible association between eNOS 4a/b polymorphism and the risk of developing type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN) 173 T2DM patients with and without DN and 101 healthy subjects with ethnic background of Kurds were examined for the frequency of eNOS variants using PCR-RFLP method. The frequency of eNOS 4a/b genotypes between T2DM and controls was not...

The clinical course of chronic renal diseases and their progression to ESRF is highly variable. Different candidate gene polymorphisms have been advocated as possible modulators of ESRF progression. Moreover, ET-1 has been suggested as a major promoting factor in renal disease. However, limited data are available regarding an association of three ET-1 SNP K198N, T- 1370G and 3A/4A with the prog...

The csg2csg tool is a minimal dependency Python3 based program to facilitate the comparison of radiation transport Monte Carlo codes. can currently parse MCNP and OpenMC geometry descriptions, it subsequently export MCNP, FLUKA, PHITS, Serpent files. This translation achieved by reading input into class system objects corresponding surfaces, cells, materials so on. are then translated ‘generic’...

Zeolite 4A, synthetic silicate, has been shown to exhibit diverse biological activities such as anti-cancer and anti-oxidant activity. In the present study, we report that the zeolite 4A may improve skin-whitening. We found that zeolite 4A inhibited melanin production in a dose-dependent manner, which has not cytotoxicity. Zeolite 4A also inhibited alpha-melanocyte-stimulating hormone (alpha-MS...

Interaction of protein synthesis initiation factors with mRNA has been studied in order to characterize early events in the eukaryotic translation pathway. Individual reovirus mRNAs labeled with 32P in the alpha position relative to the m7G cap and eukaryotic initiation factor (eIF)-4A, -4B, and -4F purified from rabbit reticulocytes were employed. It was found that eIF-4A causes a structural c...