A simple method for preparation of brush border membranes (BBM) from rat kidney using polyethylene glycol (PEG) precipitation has been described. This method avoids the use of cations for the preparation, which might alter membrane lipid composition. These preparations were assessed for enrichment of marker enzymes, contamination by subcellular structures, lipid composition and transport functi...

Preparations enriched with plasmalemmal, outer mitochondrial, or Golgi complex membranes from rat liver were subfractionated by isopycnic centrifugation, without or after treatment with digitonin, to establish the subcellular distribution of a variety of enzymes. The typical plasmalemmal enzymes 5'-nucleotidase, alkaline phosphodiesterase I, and alkaline phosphatase were markedly shifted by dig...

Lipophorin binding to the midgut of Manduca sexta larvae was characterized in a midgut membrane preparation, using iodinated larval high-density lipophorin ((125)I-HDLp-L). The iodination procedure did not change the affinity of the preparation for lipophorin. In the presence of increasing concentrations of membrane protein, corresponding increases in lipophorin binding were observed. The time-...

The aim of this study was to establish a rapid preparation of plasma membrane from adipocytes and muscle cells to detect translocated glucose transporter (GLUT) 4. A plasma membrane fraction was prepared by sequential centrifugation with buffer containing detergents, and its purity was estimated by detecting insulin receptor beta-subunit (IRbeta). After insulin stimulus, GLUT4 translocation was...

this study focuses on the preparation and modification of a thin-film composite (tfc) hollow fiber polyamide membrane fabricated by the interfacial polymerization of piperazine (pip) and trimesoyl chloride (tmc) on a porous polysulfone substrate. the effects of triethylenetetramine (teta) and silica nanoparticles (sio2) contents in the aqueous phase (as the additives) on the morphology and perf...

In this study, novel polycarbonate-titanium oxide nanotubes (PC-TNT) ultrafiltration mixed matrix membranes (MMMs) were fabricated for decontamination of Cd2+ and Cu2+ metal ions from aqueous solution. The weight percent of TNTs in the polycarbonate membrane matrix was changed from 0 to 15. The synthesized neat PC membrane and PC-TNTs MMMs were characterized with respect to structural morpholog...

The preparation of a subcellular fraction from bovine adrenal cortex is described. Characterization by electron microscopy, electron microscopic cytochemistry, and enzymatic assay indicates that this fraction is enriched plasma membranes. The preparation binds the steroidogenically active [Gin”] [W-Phe7] corticotropin, -ED amide. The specificity of this binding is demonstrated by competitive bi...