In methanogenic microbial electrolysis cells (MMCs), CO2 is reduced to methane using a methanogenic biofilm on the cathode by either direct electron transfer or evolved hydrogen. To optimize methane generation, we examined several cathode materials: plain graphite blocks, graphite blocks coated with carbon black or carbon black containing metals (platinum, stainless steel or nickel) or insolubl...

rumen is a persistent and specific ecosystem consists of bacteria, protozoa and fungus where feed fermentation takes place in it. produced hydrogen in rumen can be used in the synthesis of the volatile fatty acids and the microbial protein and its excess would be eliminated through the production of methane by methanogenesis. nutritionists have tried to find ways to decrease loss and energy and...

The objective of this study was to investigate the effects of 11 active compounds of essential oils (ACEO) on rumen fermentation characteristics and methane production. Two trials were conducted. In trial 1, ACEO (eugenol, carvacrol, citral, limonene, 1,4-cineole, p-cymene, linalool, bornyl acetate, α-pinene, and β-pinene) at a dose of 1,000 μL/L were incubated for 24 h in diluted rumen fluid w...

A study of anaerobic sediments below cyanobacterial mats of a low-salinity meltwater pond called Orange Pond on the McMurdo Ice Shelf at temperatures simulating those in the summer season (<5 degrees C) revealed that both sulfate reduction and methane production were important terminal anaerobic processes. Addition of [2-(14)C]acetate to sediment samples resulted in the passage of label mainly ...

Biochemical methane potential of four species of Ulva and Gracilaria genus was assessed in batch assays at mesophilic temperature. The results indicate a higher specific methane production (per volatile solids) for one of the Ulva sp. compared with other macroalgae and for tests running with 2.5% of total solids (196±9 L CH(4) kg(-1)VS). Considering that macroalgae can potentially be a post tre...

The potential for improving long-chain fatty acids (LCFA) conversion to methane was evaluated by bioaugmenting a non-acclimated anaerobic granular sludge with Syntrophomonas zehnderi. Batch bioaugmentation assays were performed with and without the solid microcarrier sepiolite, using 1 mM oleate as sole carbon and energy source. When S. zehnderi was added to the anaerobic sludge methane product...

[1] Despite the importance of methane for climate, it has remained difficult to measure gross rates of methane production and consumption without inducing artifacts. To remedy this, we have developed, tested, and applied a field-based CH4 pool dilution technique. Laboratory tests, sensitivity analyses, and field data indicate that this technique is robust for measuring gross rates of methane pr...

Coalbed methane (CBM) plays an important role in coal mining safety and natural gas production. In this work, The CBM potential of B2 seam in Parvadeh IV coal deposit, in central Iran, was evaluated using a combination of local regression and geostatistical methods. As there were 30 sparse methane sampling points in the Parvadeh IV coal deposit, no valid variogram was achieved for the methane c...

This study was undertaken to evaluate the efficiency of a liquid-solids separation process and microwave pretreatment, as well as anaerobic biodegradability of microwave pretreated dairy manure. Liquid-solids separation of raw dairy manure resulted in solid and liquid fractions having different properties, with the solid fractions richer in total and volatile solids content and liquid fractions...

Rates of methane production in Minnesota peats were studied. Surface (10- to 25-cm) peats produced an average of 228 nmol of CH(4) per g (dry weight) per h at 25 degrees C and ambient pH. Methanogenesis rates generally decreased with depth in ombrotrophic peats, but on occasion were observed to rise within deeper layers of certain fen peats. Methane production was temperature dependent, increas...