bacterial resistance to antibiotics is a serious problem and is increasing in prevalence world-wide at an alarming rate. the antimicrobial susceptibility patterns of 1897 gram-positive bacterial isolates were evaluated. the minimum inhibitory concentration (mic) of isolates which comprised staphylococcus aureus (927 isolates), coagulase-negative staphylococci (cns; 425 isolates), enterococcus f...

Oxacillin/methicillin-resistance is related to the mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to CNS, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biolog...

Introduction: Methicillin-Resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious hospital- and community-acquired infections. USA300 is known to be the most common cause of community-acquired infections, but recently there have been some reports on hospital-acquired infections caused by this strain. Methods: Totally 171 isolates of S. aureus were collected fro...

The aim of this study was to assess the species composition of airborne Staphylococcus spp. in public premises, to determine the methicillin resistance of the isolates and the prevalence of mecA gene, determining resistance to β-lactams. In total 65 Staphylococcus strains were isolated from 54 sites. Four strains exhibited phenotypic methicillin resistance, while the presence of mecA gene was f...

objective: methicillin-resistant staphylococcus aureus is a major cause of nosocomial and community-acquired infections. aminoglycosides are potent bactericidal agents that are often used in combination with either a β-lactam or a glycopeptide, especially in the treatment of staphylococcal endocarditis. the main mechanism of aminoglycoside resistance in staphylococci is drug inactivation by cel...

This study was conducted to determine the species distribution, antimicrobial resistance pheno- and genotypes and virulence traits of mannitol-positive methicillin-resistant staphylococci (MRS) isolated from pigs in Nsukka agricultural zone, Nigeria. Twenty mannitol-positive methicillin-resistant coagulase-negative staphylococcal (MRCoNS) strains harboring the mecA gene were detected among the ...

background staphylococcus aureus is a common pathogen in hospitals and communities. antibiotic resistance is a major public health problem. objectives the aim of this study was the determination of antimicrobial susceptibility patterns and to perform molecular detection of macrolide and lincosamide-resistance genes in clinical s. aureus isolates from kerman, iran. conclusions a high prevalence ...

The EVIGENE MRSA Detection Kit was evaluated on coagulase-negative staphylococci (CoNS) from agar plates and on staphylococci directly from positive spiked blood cultures. For the CoNS study, a total of 242 isolates were tested, and of these 237 gave valid test results. For the 237 valid tests, all gave correct mecA classification. For the blood culture procedure, a collection of 51 mecA-positi...

Absolute dependence on mecA gene as the defining standard in determining the resistance of S. aureus to methicillin became the subject of distrust by many researchers. The present study aimed to determine the frequency of mecA gene in methicillin resistant S. aureus (MRSA) isolates using polymerase chain reaction and to correlate its presence to conventional method. In this regard, two hundred ...

The staphylococcal methicillin resistance determinant, mecA, resides on a mobile genetic element, staphylococcus chromosomal cassette mec (SCCmec). The distribution of SCCmec in nature is limited to relatively few clonal complexes of related methicillin-resistant Staphylococcus aureus (MRSA). We have previously reported that some genetic backgrounds are restrictive of mecA and penicillin-bindin...