Microplate readers have been useful assistants of researchers for several decades. This work is focused on the applications of a simple absorbance microplate reader in yeast physiology research, and its advantages and limitations in comparison with alternative methods are discussed. The two main procedures involved are measuring growth curves and monitoring the pH changes of medium using two di...

The TopCountTM Microplate Scintillation Counter quantitates radioisotopes by liquid scintillation counting in standard microplate formats. Assays that previously had to be transferred from microplates to vials or to non-standard formats for counting can now be quantitated directly in microplates. TopCount can count up to 12 samples simultaneously with performance comparable to that of discrete ...

In this study, siderophore production by various bacteria amongst the plant-growth-promoting rhizobacteria was quantified by a rapid and efficient method. In total, 23 siderophore-producing bacterial isolates/strains were taken to estimate their siderophore-producing ability by the standard method (chrome azurol sulphonate assay) as well as 96 well microplate method. Production of siderophore w...

A simple and reliable method for quantification of biotinidase (EC.3.5.1.12) activity in dried blood spot was devised by a modification of the colorimetric screening test developed by Heard et al. (1984). The enzyme reaction and hemoglobin denaturation were carried out in a U-bottomed microplate. An aliquot of the reaction solution was transferred to a flat-bottomed microplate. After the coupli...

Common Western-blot imaging systems have previously been adapted to measure signals from luminescent microplate assays. This can be a cost saving measure as Western-blot imaging systems are common laboratory equipment and could substitute a dedicated luminometer if one is not otherwise available. One previously unrecognized limitation is that the signals captured by the cameras in these systems...

Background: Bradford protein assay is popular due to its ease of performance and relative sensitivity. Many researchers and laboratories in Iran use standard assay of Bradford by cuvette. No commercial kit was available for Bradford microplate assay in Iran. Meanwhile, imported Bradford commercial kits are very expensive and have a long delivery time in Iran. Till now no study or document on Br...

[1] This work explores the control of fore-arc structure on segmentation of megathrust earthquake ruptures using coastal geomorphic markers. The Arauco-Nahuelbuta region at the south-central Chile margin constitutes an anomalous fore-arc sector in terms of topography, geology, and exhumation, located within the overlap between the Concepción and Valdivia megathrust segments. This boundary, howe...

Berries and berry production are an important economic factor. Berries contain nutritive components but also pertinent non-nutritive bioactives like phenolic-type phytochemicals. It is well accepted that consumption of bioactives from berries prevent or delay chronic and degenerative diseases. Therefore, a thorough evaluation of bioactive compounds is of utmost interest to assess berry quality....

We have developed a quantitative competitive PCR (QC-PCR) assay in a microplate format for quantifying human immunodeficiency virus Type 1 (HIV-1) DNA or RNA in a broad range of source materials. Our QC-PCR assay is a modification of technique originally described by Piatak et al. (1993), which is based on the presence of a competitive internal standard containing an internal 80-bp deletion of ...

We describe a simple microplate hybridization assay for the rapid detection of the IS6110 PCR products of Mycobacterium tuberculosis from clinical cultures and from sputum specimens. The assay is based on the specific detection with a fluorescein-labeled detection probe of biotinylated PCR products which are captured on avidin coated microplate. Hybridized products with fluorescein were identif...