Microsatellites or simple sequence repeats (SSRs) are very effective molecular markers in population genetics, genome mapping, taxonomic study and other large-scale studies. Variation in number of tandem repeats within microsatellite refers to simple sequence length polymorphism (SSLP); but there are a few studies that are showed SSRs replication slippage may be occurred during in vitro amplifi...

PURPOSE Esophageal squamous cell cancer can be treated effectively by potentially curative surgery if diagnosed at an early stage. Our aim was to develop a novel molecular approach as a noninvasive test for squamous cell cancer detection and as an indicator for the prognosis of the patients. EXPERIMENTAL DESIGN Matched normal, tumor, and serum samples were obtained from 28 patients with squam...

optimization of the condition for pcr-directed sequencing of microsatellites poly adenine (a) length polymorphisms is more difficult and sensitive compared with other common sequences. replication slippage may occur for polymerase enzyme during microsatellite amplification and direct sequencing of these pcr products will be challenging for heterozygote samples. so, the aim of this study is to i...

this study was conducted in order to investigate genetic diversity and population structure of pike-perch in the northern part of iran. for this purpose, 207 adult pike-perches from four regions of the caspian sea watershed (talesh coasts, anzali wetland, chaboksar coasts and aras dam) were collected. dna was extracted and by using 15 pairs of microsatellite primers, polymerase chain reaction (...

OBJECTIVES Variation in microsatellite sequences that are dispersed in the genome has been linked to a deficiency in cellular mismatch repair system and defects in several genes of this system are involved in carcinogenesis. Our aim in this study was to illustrate microsatellite DNA alteration in esophageal cancer. MATERIALS AND METHODS DNA was extracted from formalin fixed paraffin embedded ...

The development of microsatellite DNA markers for use with butterflies and moths is extremely difficult for no apparent reason. New findings demonstrate that many lepidopteran microsatellite sequences exist in multiple copies in the genome, and have similar or almost identical flanking regions. These findings provide a compelling explanation for the low efficiency of microsatellite isolation in...

Species. Oncorhynchus mykiss. Source and Description. Microsatellite repeats were identified by sequencing clones from microsatellite-enriched libraries constructed from genomic DNA. Microsatellite markers were developed by designing primers to amplify the repeats. Primer Sequences. See Table 1. Method of Detection. PCR conditions were optimized using rainbow trout DNA (Kamloop strain) and mini...

The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) is involved in cellular defences against alkylating agents. Alterations in the MGMT gene may result in an increase in the mutation rate and risk of malignant transformation. We have previously shown that MGMT is implicated in colorectal carcinogenesis particularly in cancers which display microsatellite instability, a marker o...

Plant development consists of the initial phase of intensive cell division followed by continuous genome endoreduplication, cell growth, and elongation. The maintenance of genome stability under these conditions is the main task performed by DNA repair and genome surveillance mechanisms. Our previous work showed that the rate of homologous recombination repair in older plants decreases. We hypo...