The anther-specific gene LLA1271 isolated from lily (Lilium longiflorum Thunb.) anthers is novel and exists in two forms. The protein encoded by LLA1271 may represent an adhesin-like protein first found in higher plants. The protein contains a typical N-terminal signal peptide followed by a highly conserved repeat domain. The LLA1271 gene is temporally expressed at the phase of microspore devel...

Tobacco (Nicotiana tabacum L.) microspores at the time of mitosis are characterized by the abundant occurrence of 92and 98-kDa glycoproteins (GP92 and GP98). GP92 is a soluble protein while GP98 is bound to the insoluble microspore fraction. Both glycoproteins were isolated by affinity chromatography and SDS– PAGE and analysed by MS. Peptide sequences were determined by l-HPLC/nano-ESI–MS/MS (e...

Under specific stress treatments, the microspore can be induced in vitro to deviate from its gametophytic development and to reprogram towards embryogenesis, becoming a totipotent cell and forming haploid embryos. These can further regenerate homozygous plants for production of new isogenic lines, an important biotechnological tool for crop breeding. DNA methylation constitutes a prominent epig...

Abstract Einkorn ( Triticum monococcum L.) can be applied as a model species for cereal genomic studies due to its small genome size and high level of polymorphism. The in vitro somatic tissue culture protocol einkorn was significantly improved recently, however the androgenesis remained an unresolved research topic. Five different pre-treatments were compared study effects stress on efficiency...

The B chromosomes of maize typically undergo nondisjunction during the second microspore division (generative cell division). When the microspore nucleus contains only one B chromosome, two kinds of sperm result, one with two B chromosomes and one with no B chromosomes. The sperm with the B chromosomes preferentially fertilizes the egg cell. Previous studies of these phenomena have been limited...

Although C-type cytoplasmic male sterility (CMS-C) is one of the most attractive tools for maize hybrid seed production, the detailed regulation network of the male sterility remains unclear. In order to identify the CMS-C sterility associated genes and/or pathways, the comparison of the transcriptomes between the CMS-C line C48-2 and its isonuclear-alloplasmic maintainer line N48-2 at pollen m...

Important phenomena related to rice, such as Cytoplasmic male sterility (CMS), self-incompatibility and sex determination, are involved in sexual reproduction. For rice, the development process of male gametophyte and pollen contains several stages, including pre-meiotic anther (PMA), meiosis (MEI), tetrad (TET), uninuclear (UN) microspore, bicellular (BC) pollen, tricellular (TC) pollen, MEI, ...

INTRODUCTION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 597 Linkage intensity between the factor pairs Su su and Ta tu in maize. .................... 598 The relative frequency of crossing over between S, su and Tu tu in megaspore and microspore development. ...................................................

background:  stresses such as heat shock, starvation, or osmotic is essential to lead isolated microspores towards embryogenesis. despite the effectiveness of stresses in embryogenesis, they exert adverse effects on metabolism and growth of the regenerated plants.objectives: the effects of heat shock and 2,4-d treatment on total protein content of treated microspores, morphological and physiolo...

A simple procedure is described for the mechanical isolation of protoplasts of unfertilized and fertilized barley egg cells from dissected ovules. Viable protoplasts were isolated from ~75% of the dissected ovules. Unfertilized protoplasts did not divide, whereas almost all fertilized protoplasts developed into microcalli. These degenerated when grown in medium only. When cocultivated with barl...