Abstract Genetically engineered chymosin from the tree shrew ( Tupaia belangeri chinensis ) has been obtained and partially characterized for first time. The target enzyme was produced in Escherichia coli , strain BL21(DE3). It shown that recombinant coagulates cow milk Bos taurus ). total specific milk-clotting activity of 0.7–5.3 IMCU/mL 8.8–16.6 IMCU/mg. nonspecific proteolytic relation to b...

A gene for calf prochymosin (prorennin) has been reconstructed from chemically synthesized oligodeoxyribonucleotides and cloned DNA copies of preprochymosin mRNA. This gene has been inserted into a bacterial expression plasmid containing the Escherichia coli tryptophan promoter and a bacterial ribosome binding site. Induction of transcription from the tryptophan promoter results in prochymosin ...

Of 48 fungal isolates, 28 (including one Penicillium and three Aspergilli) secreted milk-clotting enzymes. This activity was in general related to the mycelial growth of a given isolate, but not with pH change of the culture filtrate during incubation. It was not found when mucilage was formed by isolates capable of doing so. The ratio of general proteolytic activity to milk-clotting activity v...

the objectives of this experiment were to evaluate the effects of dietary protein supplementation in lactating dairy ewe’s diets on milk coagulation properties. milk samples (n=126) were analyzed to assess the source of variation for rennet coagulation ability at the 6th (rca6) and 12th hour (rca12), milk clotting time (mct, s) and an index of milk clotting time (imct, %). in addition, data wer...