introduction cholinergic system is one of the important systems of mammalian cns. cholinergic neurons distributed in brain and spinal cord and contributed to principal functions like: consciousness, learning and memory, and motor control. in this study we investigated the differentiation potentiality of mouse embryonic stem cells toward cholinergic neurons. the aim of this study was to evaluate...

background: recently, embryonic stem (es) cells have become very important resources in basic medical researches. these cells can differetiate into derivatives of all primary germ layers. objectives: in order to isolate embryonic stem cells in vitro, the blastocyst were cultured and the morphological aspects, population doubling time, alkalin phosphatse and differentiation properties of the cel...

Background:Differentiation of Embryonic Stem Cells into Oocyte-like cells in vitro is challenging. Successful derivation of oocyte from stem cells can provide an alternative source for curing ovogenesis problems. The current study aims to demonstrate a new protocol with two different types of media for differentiating embryonic stem cells (ESCs) into oocyte-like cells ...

Cell replacement as a route to treat cellular disease and injury is currently limited by the availability of suitable donor cell populations, but recent results with mouse embryonic stem cells suggest that isolated human pluripotent cells could provide a source of cells for transplantation and gene therapy.

introduction: considering the importance of co-culture in differentiation of embryonic stem cells, the aim of this study was evaluation of the effect of co-culturing fetal liver stroma cells with p19 cells on the line of differentiation. materials and methods: for this purpose, p19 cells were cultured directly in semisolid medium. these cells proliferated and primarily differentiated to colonie...

Background: Recombination Activating Genes (RAG) mutated embryonic stem cells are (ES) cells which are unable to perform V (D) J recombination. These cells can be used for generation of immunodeficient mouse. Creating biallelic mutations by CRISPR/Cas9 genome editing has emerged as a powerful technique to generate site-specific mutations in different sequences. Ob...