The objective of this study was to determine the thermal resistance of spores of Bacillus anthracis and potential surrogates. The heat resistance of spores suspended in buffer (pH 7.0 or 4.5), milk, or orange juice was determined at 70, 80, and 90 degrees C. D-values for B. anthracis strains Sterne, Vollum, and Pasteur ranged from < 1 min at 90 degrees C to approximately 200 min at 70 degrees C...

Five original strains of entomopathogenic bacteria with insecticidal activity against mosquito larvae of the genera Aedes, Culex and Anopheles have been isolated from natural conditions in Armenia and characterized. According to morphological, physiological and biochemical parameters, all isolates were identified as Bacillus thuringiensis spp. israelensis (Bti). High larvicidal activity has bee...

Bacillus anthracis can infect both livestock and humans. The importance of the treatment of the disease in humans has been underscored by the bioterrorism events of 2001 in the United States. The presence of PA and B/C genes (pX01 and pX02 plasmids) as well as susceptibility to several antimicrobial substances was determined in 11 strains of Bacillus anthracis isolated during two recent epizoot...

in the present study, we aimed to screen indigenous rhizospheric bacillus strains, capable of producing antiphytopathogenic and plant growth promoting traits. isolate cts-b19 and cts-g24 exhibited quite noticeable antagonistic activity initially against fusarium oxysporum f. sp. ciceri and rhizoctonia bataticola, and, later, against a panel of phytopathogens. partial 16s rrna sequence analysis ...

Gum of Butea monosperma is used to treat microbial and fungal infections in folk medicine. To validate this use, the in- vitro antimicrobial activity of petroleum ether and alcoholic extract of Butea monosperma gum was evaluated against various microbial strains such as Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Salmonella typhimurium , Pseudomonas aeuriogenosa, Esch...

Bacillus strains have been previously shown to be able to sporulate in biofilms. In order to estimate if biofilms could be a significant source of food contamination with spores, we investigated the ability of Bacillus strains to form biofilms. Most of the tested strains were able to form biofilms. We then analyzed four biofilm-producing strains for their ability to sporulate within biofilms an...

The methanolic extract of the leaf-stalk of curcuma longa LINN, was tested for its minimum Inhibitor concentration (MIC) against Gram positive-staphylococcus aureus, Bacillus pumilus, Bacillus subtilis, klebsiella pnemoniae, bacillus cereus, streptococcus pneumoniae, Lactobacillus arabinosus and gram negative E.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium...

Five strains of Gram-positive, endospore-forming, moderately halotolerant bacteria were studied taxonomically. Four were isolated from Vietnamese fish sauce and one from the Gulf of Mexico. Phylogenetic analysis based on 16S rRNA gene sequences showed that these strains clustered within the radiation of the genus Bacillus but separately from recognized Bacillus species. DNA G+C composition of t...

We investigated whether Bacillus spp., newly isolated from Korean traditional food resources, influence the resistance of hosts to foodborne pathogens, by using Caenorhabditis elegans as a surrogate host model. Initially, we selected 20 Bacillus spp. that possess antimicrobial activity against various foodborne pathogens, including Staphylococcus aureus. Among the selected strains, six strains ...

The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An-oligonucleotide probe based on the area approximately between position 186 and 198 (Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology g...