BACKGROUND Characterization of fusion gene transcripts in leukemia that result from chromosome translocations provides valuable information regarding appropriate treatment and prognosis. However, screening for multiple fusion gene transcripts is difficult with conventional PCR and state-of-the-art real-time PCR and high-density microarrays. METHODS We developed a multiplex reverse transcripti...

Abstract Background Respiratory tract infections are the most common that lead to morbidity and mortality worldwide. Early recognition precise diagnosis of microbial etiology is important treat LRTIs promptly, specifically effectively. Objectives To establish a method based on multiplex reverse transcription (MRT)-PCR MassARRAY technology for simultaneous detection 27 respiratory pathogens expl...

background : avian influenza virus (aiv) infection is a major cause of bird and human morbidity and mortality. we aimed to evaluate a specific and sensitive multiplex rt-pcr that can simultaneously detect influenza type a viruses and differenti­ate the two most important subtypes of avian influenza viruses h7 and h9 subtypes. methods : a multiplex reverse transcriptase-polymerase chain reaction...

This study was carried out in bean seeds Isparta and Antalya provinces to determine Bean common mosaic virus (BCMV) 2016. For this purpose; 106 seed samples were collected from the research area. Seed used for biological, serological, molecular methods. The incidence, as a percentage of BCMV, found 87.73% by DAS-ELISA 90.56% Immunocapture Reverse Transcriptase-Polymerase Chain Reaction (IC-RT-P...

Objective: To establish the accuracy of computed tomography(CT) chest in diagnosis COVID-19 pneumonia by taking reverse transcriptase-polymerase chain reaction(RT-PCR) as a reference standard and to analyze discordant CT RT-PCR results.Materials Methods: A retrospective cross-sectional study patients presented tertiary health care hospital Punjab, Pakistan for examination with suspicion from Ap...

The present study was carried out to detect the circulating Newcastle disease virus (NDV) from various field outbreaks in Assam during 2018-2021. Nine hundred ninety-two clinical and post mortem samples were collected backyard commercial poultry subjected molecular detection by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). NDV could be detected 445 (n=757, 58.78%) tissue 53 (n=235, ...

A multiplex reverse transcription-PCR-enzyme hybridization assay (RT-PCR-EHA; Hexaplex; Prodesse Inc., Waukesha, Wis.) was used for the simultaneous detection of human parainfluenza virus types 1, 2, and 3, influenza virus types A and B, and respiratory syncytial virus types A and B. One hundred forty-three respiratory specimens from 126 patients were analyzed by RT-PCR-EHA, and the results wer...

Rates of detection of rotavirus were compared by diagnostic assay and specimen type. For bulk stools, rates of detection by reverse transcriptase PCR (RT-PCR) and enzyme immunoassay (EIA) were similar, but 18% of healthy controls tested positive by RT-PCR. Testing of bulk stools by EIA appears to be optimum for rotavirus surveillance.

Reverse transcriptase PCR (RT-PCR) consistently detected bovine leukemia virus transcripts in fresh cells, and competitive RT-PCR enumerated these transcripts. The detection of transcripts in limited numbers of tumor cells indicated that expression occurs in a minority of cells. The data suggest that individual cells contain hundreds of copies of the tax/rex transcript in vivo.