Mycoplasma gallisepticum (MG) is a reproductive/respiratory disease in poultry implicated in suboptimum egg production and decreased hatchability. Commercial layer hens raised in a controlled environment were inoculated with the S6 strain of MG at 10 wk of age. Egg production and selected egg and egg quality parameters were quantitated over the entire lay cycle for inoculated and control birds....

In order to identify antigenic proteins of Mycoplasma gallisepticum, monoclonal antibodies (MAbs) against virulent M. gallisepticum R strain were produced in mice. MAb 35A6 was selected for its abilities to inhibit both growth and metabolism of M. gallisepticum in vitro. The MAb recognized a membrane protein with an apparent molecular mass of 120 kDa. The corresponding gene, designated the mgc3...

Colonization of the avian respiratory tract with Mycoplasma gallisepticum results in a profound inflammatory response in the trachea, air sacs, conjunctiva, and lungs. A live attenuated M. gallisepticum vaccine strain, GT5, was previously shown to be protective in chickens upon challenge; however, the mechanisms by which this vaccine and others confer protection remain largely unknown. The curr...

While the genomes of many Mycoplasma species have been sequenced, there are no collated data on translational start codon usage, and the effects of alternate start codons on gene expression have not been studied. Analysis of the annotated genomes found that ATG was the most prevalent translational start codon among Mycoplasma spp. However in Mycoplasma gallisepticum a GTG start codon is commonl...

De Sá S.G., Pinheiro Junior J.W., Vilela S.M.O., Moraes E.P.B.X., Albuquerque P.P.F., Porto W.J.N., Ferreira D.R.A. & Mota R.A. 2015. Occurrence and risk factors assessment associated with Mycoplasma gallisepticum (MG) infection in chickens in the semiarid region of Pernambuco, Brazil. Pesquisa Veterinária Brasileira 35(6):531535. Laboratório de Doenças Infecto-Contagiosas dos Animais Doméstico...

Immunofluorescence can be used to demonstrate mixed species within a mycoplasma culture; however, it may fail to do so if one species is present in very low numbers. To enhance the detection of minor components in such mixtures, a technique was developed, based on the growth inhibition test, whereby the growth of the major component in a mixed culture was inhibited, thus permitting the minor co...

Mycoplasma gallisepticum is the major mycoplasmal pathogen in poultry. The present study was undertaken to evaluate the efficacy of six commonly used antimicrobials, including difloxacin, enrofloxacin, ciprofloxacin, doxycycline, gentamicin, and valnemulin, against Mycoplasma gallisepticum, using a Monte Carlo simulation based on the pharmacokinetics-pharmacodynamic (PKPD) theory. The ratio of ...

Antigens for Mycoplasma gallisepticum were prepared from organisms propagated in media with and without dextrose supplementation. The antigens made from organisms produced in medium enriched with dextrose were less sensitive than the others in slide agglutination tests.

In this study, the authors examined the technical performance of culture methodology using specific media: Mycoplasma isolation media of pleuropneumonia-like organisms (PPLO) broth and PPLO agar. Digitonin sensitivity, growth inhibition, the serum plate agglutination test, a commercially available enzyme-linked immunosorbent assay (ELISA) and a commercially available simplex polymerase chain re...

The present work was conducted to study the seroprevalence of Salmonella, Mycoplasma gallisepticum (MG), and Mycoplasma synoviae (MS) infection in backyard chickens located in Entre Ríos, Argentina, over 3 periods of time. A total of 2,441 sera samples were collected from backyard chickens belonging to 256 family farms in 16 counties in the state of Entre Ríos from January to May 2003 (first pe...