by the decrease in incorporation of labelled methyl groups from enzymically synthesized S-adenosylmethionine into basic protein by spinal-cord protein methylase activity (Table 1). Electrophoresis of the labelled protein precipitate on acetic acidhrea-polyacrylamide gels (Deibler et al., 1972), followed by autoradiography of the gels, indicated that only the myelin basic protein was detectably ...

Guinea pig myelin basic protein has been chromatographed on carboxymethylcellulose at alkaline pH to yield five purified components, each of which was shown to be identical with one of the several components which have previously been observed electrophoretically at alkaline pH. Each purified chromatographic component was stable upon electrophoresis at alkaline pH. No differences in molecular s...

Myelination was studied in aggregating cell cultures derived from mechanically 15- to 16-day fetal rat brains. Myelin basic protein (MBP) and myelin-associated glycoprotein (MAG) were localized immunocytochemically 20-micrometers-thick Vibratome 1-micrometer- thick Epon sections at 15, 20, 25, 30 days vitro. The occurrence of these proteins correlated with the ultrastructural appearance oligode...

Studies of lipid-protein interactions in double-reconstituted systems involving both integral and peripheral or lipid-anchored proteins are reviewed. Membranes of dimyristoyl phosphatidylglycerol containing either myelin proteolipid protein or cytochrome c oxidase were studied. The partner peripheral proteins bound to these membranes were myelin basic protein or cytochrome c, respectively. In a...