Preliminary work revealed that nitrate reductase in crude extracts prepared from leaves of certain corn genotypes as well as soybeans could utilize NADPH as well as NADH as the electron donor. Isoelectric focusing and diethylaminoethyl cellulose chromatography confirmed previous findings that NADH and NADPH activities could not be separated, which suggests the involvement of a single enzyme. Ni...

Striatal and cortical neurons containing NADPH-diaphorase [NADPH-d(+)] are highly vulnerable to excitotoxicity that is induced by activation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)- or kainate-sensitive glutamate receptors. This has been attributed to Ca2+ entry through AMPA/kainate receptors in NADPH-d(+) neurons. In this study, we applied single cell RT-PCR techniqu...

Background NADPH provides reducing power for macromolecule synthesis and antioxidant defense. The pathways used to make NADPH under different physiological circumstances remain unclear. In growing cells, much NADPH production is coupled to nucleotide synthesis via the oxidative pentose phosphate and folate pathways. Here we examine, using isotope tracers and flux analysis, NADPH production rout...

Dikalov SI, Li W, Doughan AK, Blanco RR, Zafari AM. Mitochondrial reactive oxygen species and calcium uptake regulate activation of phagocytic NADPH oxidase. Am J Physiol Regul Integr Comp Physiol 302: R1134–R1142, 2012. First published March 21, 2012; doi:10.1152/ajpregu.00842.2010.—Production of superoxide (O2 ) by NADPH oxidases contributes to the development of hypertension and atherosclero...

OBJECTIVE NADPH, a substrate for the superoxide-producing enzyme NADPH oxidase, produces vasodilation in the cerebral circulation. However, the mechanisms of the effect have not been fully elucidated. We used a peptide inhibitor of NADPH oxidase (gp91ds-tat) and null mice lacking the gp91phox subunit of NADPH oxidase to examine the mechanisms of the cerebrovascular effects of exogenous NADPH. ...

The present study was first aimed at a complete steady-state kinetic analysis of the reaction between guaiacol (2-methoxyphenol) and the myeloperoxidase (MPO)/H2O2 system, including a description of the isolation and purification of MPO from human polymorphonuclear neutrophil cells. Secondly, the overall reaction of the oxidation of NADPH, mediated by the reactive intermediates formed from the ...

Nicotinamide adenine dinuclotide phosphate reduced form (NADPH), an electron carrier, is a kind of antioxidants against the oxidative stress in the mitochondria. The major resources of NADPH are the pentose phosphate pathway and photosynthesis. This paper will focus on the structure, formation, reaction, detection and stability of NADPH. Jingru Liu NADPH 3 Introduction: Recently, reactive oxyge...

Apocynin has been used as an efficient inhibitor of the NADPH oxidase complex in experimental studies. NADPH oxidase was originally identified immune cells as playing an important microbicidal role. In cerebral ischemia, inflammation is increasingly being recognized as contributing negatively to neurological outcome, with NADPH-oxidase as an important source of superoxide. Recently, several for...

Oxidative deformylation of 4-hydroxy[14C]methylene-5alpha-cholest-7-en-3-one and oxidative demethylation of [30,31-14C]4,4-dimethyl-5alpha-cholest-7-en-3beta-ol by rat liver microsomes have been compared with regard to the manner in which electrons are introduced from both NADH and NADPH. Evidence suggests that NADH and NADPH support oxidation of both substrates via separate routes of electron ...

In this study, the innervation of the urethral muscle in adult male pigs was investigated using combined NADPH-diaphorase (NADPH-d) histochemistry and immunocytochemistry. Nerve fibres supplying the urethral muscle were found to show NADPH-d activity and they also expressed immunoreactivity to catecholamine synthesising enzymes including tyrosine hydoxylase (TH) and dopamine-beta-hydroxylase (D...