Cellular processes take place over many timescales, prompting the development of precision measurement technologies that cover milliseconds to hours. Here we describe ArrayG, a bipartite fluorogenic system composed of a GFP-nanobody array and monomeric wtGFP binders. The free binders are initially dim but brighten 15 fold upon binding the array, suppressing background fluorescence. By balancing...

Background: Single domain antibodies from camel heavy chain antibodies (VHH or nanobody), are advantages due to higher solubility, stability, high homology with human antibody, lower immunogenicity and low molecular weight. These criteria make them candidates for production of engineered antibody fragments particularly in transgenic animals. Objective: To study the development of transgenic ch...

We present catalyst-free “green” site-selective protein bioconjugations that utilise aldol condensations and are compatible with click chemistries, construct a nanobody-derived bioconjugate capable of selectively labelling prostate cancer cells.

Wet age-related macular degeneration (wet AMD) is the most common cause of blindness, and chronic intravitreal injection anti-vascular endothelial growth factor (VEGF) proteins has been dominant therapeutic approach. Less a prolonged inter-injection interval are main drivers behind new wet AMD drug innovations. By rationally engineering surface residues model anti-VEGF nanobody, we obtained ser...

DNA nanostructures have become an important and powerful tool for studying protein function over the last 5 years. One of the challenges, though, has been the development of universal methods for patterning protein complexes on DNA nanostructures. Herein, we present a new approach for labeling DNA nanostructures by functionalizing them with a GFP nanobody. We demonstrate the ability to precisel...

Protein-protein interactions are the basis of all processes in living cells, but most studies of these interactions rely on biochemical in vitro assays. Here we present a simple and versatile fluorescent-three-hybrid (F3H) strategy to visualize and target protein-protein interactions. A high-affinity nanobody anchors a GFP-fusion protein of interest at a defined cellular structure and the enric...

background: single domain antibodies from camel heavy chain antibodies (vhh or nanobody), are advantages due to higher solubility, stability, high homology with human antibody, lower immunogenicity and low molecular weight. these criteria make them candidates for production of engineered antibody fragments particularly in transgenic animals. objective: to study the development of transgenic chi...

Single-domain antibodies, known as nanobodies, have great potential biorecognition elements for sensors because of their small size, affinity, specificity, and robustness. However, facile efficient methods nanobody immobilization are sought that retain maximum functionality. Herein, we describe the direct nanobodies on gold by exploiting a modified cysteine strategically positioned at C-termina...

Recent clinical trials have shown that adoptive chimeric antigen receptor (CAR) T cell therapy is a very potent and possibly curative option in the treatment of B cell leukemias and lymphomas. However, targeting a single antigen may not be sufficient, and relapse due to the emergence of antigen negative leukemic cells may occur. A potential strategy to counter the outgrowth of antigen escape va...