Gesellschaft für Biotechnologische Forschung, Mascheroder Weg, D-3300 Braunschweig-Stöckheim, Bundesrepublik Deutschland Z. Naturforsch. 42c, 69—72 (1987); received August 21/September 22, 1986 Agrobacterium rhizogenes, Alkaloids, Nicotine, Cytisine, Nicotiana, Spartium The growth of root hairs from stems of Nicotiana tabacum and Spartium junceum was induced by Agrobacterium rhizogenes. Stem ro...

For Nicotiana, with 75 naturally occurring species (40 diploids and 35 allopolyploids), we produced 4656bp of plastid DNA sequence for 87 accessions and various outgroups. The loci sequenced were trnL intron and trnL-F spacer, trnS-G spacer and two genes, ndhF and matK. Parsimony and Bayesian analyses yielded identical relationships for the diploids, and these are consistent with other data, pr...

Epitrix hirtipennis (Melsheimer, 1847) (Coleoptera: Chrysomelidae: Galerucinae: Alticini), a leaf beetle native to North America, is recorded for the first time from Hungary. Several specimens were found on tobacco (Nicotiana tabacum L.) plantations in eastern and central parts of country. Its life cycle importance as pest are briefly discussed.

An approximately 135-bp sequence called the A1/A2 repeat was isolated from the transcribed region of the 26-18S rDNA intergenic spacer (IGS) of Nicotiana tomentosiformis. Fluorescence in situ hybridization (FISH) and Southern blot analysis revealed its occurrence as an independent satellite (termed an A1/A2 satellite) outside of rDNA loci in species of Nicotiana section Tomentosae. The chromoso...

Nicotiana alata pollen tubes are a widely used model for studies of polarized tip growth and cell wall synthesis in plants. To better understand these processes, RNA-Seq and de novo assembly methods were used to produce a transcriptome of N. alata pollen grains. Notable in the reconstructed transcriptome were sequences encoding proteins that are involved in the synthesis and remodelling of xylo...

Efficient plastid transformation has been achieved in Nicotiana tabacum using cloned plastid DNA of Solanum nigrum carrying mutations conferring spectinomycin and streptomycin resistance. The use of the incompletely homologous (homeologous) Solanum plastid DNA as donor resulted in a Nicotiana plastid transformation frequency comparable with that of other experiments where completely homologous ...

Shifts in spectral distribution of light were determined within and below a canopy of field-grown burley tobacco (Nicotiana tabacum L. cv. Burley 21). The leaves transmitted much far red light relative to red and blue light. Thus, shaded leaves received more far red light, relative to red and blue, than was received by unshaded leaves. Under field conditions, tobacco plants within rows grew tal...