We developed an efficient synthesis of monodisperse, highly surface charged, high refractive index ZnS spherical particles by using a gel-sol method. Concentrated solutions of zinc-ammonia-NTA (nitrilotriacetic acid) were reacted with thioacetamide in the presence of gelatin which stabilized the growing particles. We dramatically increased the particle surface charge density by condensing silic...

Self-assembly of viral coating proteins encapsulating functional nanoparticles provides a new class of biomaterials with robust chemical and physical properties for potential applications in functional imaging, and therapeutic or diagnostic agent delivery. Herein, a straightforward method is demonstrated for efficient encapsidation of magnetic nanoparticles into the engineered virus-like partic...

Since immobilized metal ion affinity chromatography (IMAC) was first reported, several modifications have been developed. Among them, Ni(2+) immobilized by chelation with nitrilotriacetic acid (NTA) bound to a solid support has become the most common method for the purification of proteins carrying either a C- or N-terminal histidine (His) tag. Despite its broad application in protein purificat...

An enrichment culture capable of naphthalene mineralization reduced Fe(III) oxides without direct contact in anaerobic soil microcosms when the Fe(III) was placed in dialysis membranes or entrapped within alginate beads. Both techniques demonstrated that a component in soil, possibly humic materials, facilitated Fe(III) reduction when direct contact between cells and Fe(III) was not possible. T...

Priming of the liver for ethanol-induced injury, by nutrients such as polyunsaturated fat and iron, plays a key role in alcoholic liver disease. The objective of this work was to evaluate the effect of the combination of Fe-nitrilotriacetic acid (Fe-NTA) and arachidonic acid (AA) on the viability of HepG2 cells (E47 cells) transfected to express human CYP2E1. Cells were plated, preloaded with a...

This article describes an experimental protocol using electrospray-ion mobility-mass spectrometry (ES-IM-MS) and energy-resolved threshold collision-induced dissociation (TCID) to measure the thermochemistry of negatively-charged [amb+M(II)+NTA]- ternary complexes into two product channels: [amb+M(II)] + NTA or [NTA+M(II)]- amb, where M = Zn Ni is nitrilotriacetic acid. The contain one alternat...

For nearly three decades, zero-valent iron (ZVI) has been used in wastewater treatment and groundwater soil remediation. ZVI can degrade contaminants by reactions of adsorption, redox, co-precipitation. It also react with oxidants like hydrogen peroxide, persulfate, ozone to produce highly reactive radicals that rapidly remove even mineralize organic contaminants. However, the application is li...

Pulse-dipolar EPR is an appealing strategy for structural characterization of complex systems in solution that complements other biophysical techniques. Significantly, the emergence genetically encoded self-assembling spin labels exploiting exogenously introduced double-histidine motifs conjunction with CuII-chelates offers high precision distance determination nonpermissive to thiol-directed l...

This study investigated the removal of sulfur and iron from shungite rocks through different methods after fine grinding: flotation, magnetic separation, microwave treatment, chemical leaching. In this work, first, a mineralogical was conducted. The carbon, silica, iron, compositions in as-received were 45.4%, 38.3%, 4.6%, 2.4%, respectively. grade 1.4% obtained. wet high-gradient separation at...