نتایج جستجو برای: nitrocellulose
تعداد نتایج: 1717 فیلتر نتایج به سال:
BACKGROUND This study was undertaken to establish the characterization of cultured oral mucosal epithelium and introducing them as an alternative source for reconstruction of ocular surface disease. METHODS Human oral epithelial cells were cultured on simple media (DMEM/HF12) as control and co-cultured on mitomycin C-treated 3T3 feeder layer, on the amniotic membrane (AM) without nitrocellulo...
To enable enhanced paper-based diagnostics with improved detection capabilities, new methods are needed to immobilize affinity reagents to porous substrates, especially for capture molecules other than IgG. To this end, we have developed and characterized three novel methods for immobilizing protein-based affinity reagents to nitrocellulose membranes. We have demonstrated these methods using re...
Introducing deterrents improves the thermal stability of nitrocellulose propellant surface, but is accompanied with inevitable problems, such as migration, residue, smoke flame, and so on. In this paper, sodium carboxymethyl function groups were chemically grafted to molecular chains by reaction denitration following etherification, which provided stability, flame suppression ion without deterr...
Photoaffinity labeling with 8-azidoadenosine 3':5'-monophosphate is a highly selective method for probing the cAMP-binding sites of the regulatory subunits of cAMP-dependent protein kinase and for identifying specific residues that are in close proximity to the cAMP-binding sites. The cAMP-binding site of a mutant RI-subunit has been characterized here and contrasted to the native RI-subunit. T...
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