In this review we describe what is recently known about the origin of free nucleic acids in human circulation, which processes can cause the elevations of their total concentrations and the alterations in the ratios among different types of circulating nucleic acids in human plasma and serum. We focus on the inflammatory response and apoptosis with regard to changes in the quantity and quality ...

The non-covalent binding of ethidium monoazide to nucleic acids is entirely analogous to that of ethidium (binding constant approximately 2-3 X 10(5) M). The ethidium monoazide can be photochemically covalently linked to nucleic acids in high yield, up to 75%, by long wavelength light. The fluorescence of ethidium monoazide and ethidium crosslinked to nucleic acids show the same environmental s...

THE ROLE OF THYMONUCLEIC ACID IN NORMAL CELLS Chromosomes and Genes Cytoplasmic Nucleic Acids Protein Synthesis Bacteria and Filterable Viruses THE ROLE OF THYMONUCLEIC ACID IN NEOPLASTIC CELLS The Significance of Nucleic Acids in the Somatic Mutation Theory of Etiology Chemical Evidence of Disturbance of Nucleic Acids in Tumors Histochemical and Cytochemical Evidence of Disturbance of Thymonuc...

A simple and ultrasensitive procedure for non-labeling detection of nucleic acids is described in this study. It is based on the photoelectrochemical detection of target nucleic acids by forming a nucleic acid/photoreporter adduct layer on an ITO electrode. The target nucleic acids were hybridized with immobilized oligonucleotide capture probes on the ITO electrode. A subsequent binding of a ph...

Nucleic acids are responsible for the storage of genetic information and directly participate in gene replication, transcription and expression, and thereby the control of nucleic acids leads to the regulation of genetic information flow and gene expression. Meanwhile, many non-coding RNAs are involved in signal transduction directly. Moreover, nucleic acid-based therapeutic strategies have bee...