The fraction of the adenovirus type 5 genome expressed as mRNA in a rat cell line, 5RK, transformed by the HsuI-G restriction fragment was analyzed by saturation hybridization using separated strands of 32P-labeled HindIII-G fragment. It was found that 45 to 50% of G fragment (about 8 X 10(5) daltons) is expressed as mRNA. The size of polyadenylic acid-terminated virus-specific cytoplasmic and ...

The cellular response to hypoxia is regulated by hypoxia-inducible factor-1α and -2α (HIF-1α and -2α). We have discovered that filamin A (FLNA), a large cytoskeletal actin-binding protein, physically interacts with HIF-1α and promotes tumor growth and angiogenesis. Hypoxia induces a calpain-dependent cleavage of FLNA to generate a naturally occurring C-terminal fragment that accumulates in the ...

In mammalian cells, the Golgi apparatus undergoes extensive fragmentation during apoptosis. p115 is a key vesicle tethering protein required for maintaining the structural organization of the Golgi apparatus. Here, we demonstrate that p115 was cleaved during apoptosis by caspases 3 and 8. Compared with control cells expressing native p115, those expressing a cleavage-resistant form of p115 dela...

This report concerns a set of sea urchin egg and embryo transcripts complementary to a single-copy region of a cloned DNA fragment (Sp88). Three distinct 16-cell embryo polysomal RNA species were found to hybridize with this fragment. These RNAs are about 1700, 3000, and 4000 nucleotides (nt) in length, and the same species were identified in unfertilized eggs. A significant fraction of all thr...

The unfolded protein response is the mechanism by which cells control endoplasmic reticulum (ER) homeostasis. Under normal conditions, UPR not activated; however, under certain stresses, such as hypoxia or altered glycosylation, can be activated due to an accumulation of proteins. activation involves three signaling pathways, IRE1, PERK and ATF6, all play vital roles in returning homeostasis le...

Intradural disk rupture has been reported [1-5] . In most cases disk herniation in the lumbar region occurs on one side or the other of the strong posterior longitudinal ligament; seldom is the central part of the ligament perforated . Very rarely , a disk fragment ruptures through the anterior aspect of the spinal dura to project intrathecally [1] . Continued negative pressure is not necessary...

The hamster intergeniculate leaflet (IGL), part of the circadian rhythm regulatory system, has very extensive interconnections with subcortical visual nuclei. The present investigation describes IGL connections with the hamster diencephalon and telencephalon and compares them with ventral lateral geniculate nucleus (VLG) connections and retinal projections. Connections of the geniculate nuclei ...

Fission fragment mass-angle correlations and mass ratio distributions have been measured for the reactions 16O+186Os, 24Mg+178Hf, 34S+168Er, and 48Ti+154Sm, forming the 202Po composite nucleus, at near barrier energies. Systematic analysis based on the expected dependence of the variance of the mass distribution on the angular momentum and temperature of the compound nucleus indicate that the t...

The immunocytochemical distribution of [Leulenkephalin and an adrenal enkephalin precursor fragment (BAM-22P) immunoreactivity was investigated in the diencephalon and brainstem of rats pretreated with relatively high doses of colchicine (300-400 pgil0 pl intracerebroventricularly). The higher ranges of colchicine pretreatment allowed the visualization of extensive enkephalincontaining systems ...