All possible haploid and diploid genotypes were created for each pair of four beneficial mutations (one in each of ERG3, ERG5, ERG6 and ERG7, main text Table 5). Each mutation was initially isolated in the BY4741 haploid background (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) and given a Beneficial Mutation Nystatin (BMN) strain number [1]. Each BMN strain was mated to BY4739 (MATα leu2Δ0 lys2Δ0 ura3Δ0)...

The plasma pharmacokinetics of multilamellar liposomal nystatin were studied in normal, catheterized rabbits after single and multiple daily intravenous administration of dosages of 2, 4, and 6 mg/kg of body weight, and drug levels in tissues were assessed after multiple dosing. Concentrations of liposomal nystatin were measured as those of nystatin by a validated high-performance liquid chroma...

In total, 14 yeast strains originating from patients with dermatomycoses, and 7 control strains (isolates from strain collections and collaborative ring trials) were investigated regarding their in vitro susceptibility to the polyene antifungal agent nystatin. Testing was performed using a broth microdilution assay based on the standardized method of susceptibility testing of yeasts per EUCAST ...

In general, attempts to isolate strains of Candida albicans resistant to the commonly employed antifungicide nystatin have been unsuccessful (M. L. Littman, M. A. Pisano, and R. M. Lancaser, Antibiotics Ann. 1957-58, p. 981, 1958; C. K. J. Paniker, P. V. Kurup, and K. Indira Devi, Indian J. Med. Res. 51:846, 1963). A few strains, however, have yielded variants exhibiting threeto fourfold resist...

Kinetic data on potassium release from and killing of Candida albicans by the four polyene antibiotics amphotericin B, amphotericin B methyl ester hydrochloride, nystatin, and nystatin methyl ester hydrochloride are presented. The nystatins were relatively more effective than the amphotericins in causing potassium release rather than killing. These data suggest that the aqueous channels or pore...

background: denture stomatitis is a common oral lesion following the use of ill-fitting dentures. a layer of tissue conditioner is usually used to improve adaptation of the denture. these liners can support the in vivo adhesion and colonization of the oral candida . the aim of this study was to evaluate the efficacy of the two common antifungal agents mixed with tissue conditioner against candi...

background: artemisia is a herbal plant that its anticandidal effect has been investigated in different studies. the aim of this study was to assess the effect of artemisia sieberi mouth wash on denture stomatitis.methods: this double blind clinical trial study was performed on 30 patients with denture stomatitis (type ii, iii). they were randomly divided into two groups. the patients in contro...

We have monitored the ligand binding of the bovine hippocampal 5-HT1A receptor following treatment with the sterol-binding antifungal antibiotic nystatin. Nystatin considerably inhibits the specific binding of the antagonist to 5-HT1A receptors in a concentration-dependent manner. However, the specific agonist binding does not show significant changes. Fluorescence polarization measurements of ...

Haldane's sieve posits that the majority of beneficial mutations that contribute to adaptation should be dominant, as these are the mutations most likely to establish and spread when rare. It has been argued, however, that if the dominance of mutations in their current and previous environments are correlated, Haldane's sieve could be eliminated. We constructed heterozygous lines of Saccharomyc...

Liposomal carriers, eminent for their potential in topical drug delivery have been chosen to help embedding of nystatin molecules in the skin layers. The objective of present study was to develop and carry out rheological investigations of nystatin liposomal gel by using optimization technology in terms of 2 factorial design. In this study attempts have been made to formulate liposomal dispersi...