Background: Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of cumulus cells on survival,in vitro maturation,fertilization and subsequent developmental capacity of mouse germinal vesicle stage oocytes during vitrification. Materials and...

objective(s) cigarette and nicotine enhances embryogenesis, fertility, pregnancy loss and ultrastructure alterations of oocyte. this study was performed to determine the effect of daily supplementation of vitamin e on oocytes apoptosis in nicotine-treated mice. materials and methods in this experimental study, 24 nmari adult female mice were randomly allocated into four experimental groups. for...

Background: Oocyte vitrification and assisted oocyte activation have increasingly important implications in assisted reproductive technology. However, an important area of concern with matured oocyte cryobiology is that elements of oocytes intimately involved in metaphase-II arrest may be modified by cryopreservation. In a comparison between different cellular characteristics of unvitrified, vi...

objective: this study evaluated in vitro maturation (ivm) of oocytes in the germinal vesicle (gv) stage in stimulated intracytoplasmic sperm injection (icsi) cycles. materials and methods: a total of 26 women, aged 18 -37 years, who were candidates for icsi at the fatemeh zahra infertility and health reproductive research center in 2007 were recruited for this study. we used the standard long p...

Background: This study was conducted in order to determine whether BCB staining performed on immature bovine oocytes to assess G6pdhlevel has an impact on ivf/ivp technology in bovine embryos. Both spermatozoa and oocytes metabolize glucose through the pentose phosphate pathway (PPP), and NADPH appears necessary for gamete fusion. Activity of glucose 6-phosphate dehydrogenase (G6PDH), the key e...

Background: Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. Method: A total of 200 germinal vesicle (...

introduction: superovulation produces complications in some patients, so invitro maturation of oocytes is used to decrease or eliminate these complications and improve ivf. moreover, ivm is used for different aspects of reproductive researches. slaughterhouse ovaries are the main source of oocytes for ivm and ivf studies. different media has been introduced and experimented for in vitro maturat...

The mammalian ovary is a large source of oocytes organized into follicles at various stages folliculogenesis. However, only limited number them can be used for in vitro embryo production (IVEP), while most have yet to complete growth and development attain full meiotic embryonic developmental competence. While the primordial ovarian cortex has potential produce mature oocytes, it still an exper...