eQTL: expression Quantitative Trait Loci; GAD: Genetic Association Database; GtEx: Genotype-tissue Expression; HapMap: Haploid Map; HGNC: Human Genome Nomenclature Committee; HPRD: Human Protein Reference Database; HPA: Human Protein Atlas; MOPED: Model Organism Protein Expression Database; PheGenI: Phenotype-Genotype Integrator; ORF: Open Reading Frame; RefSeq: Reference Sequence; SNP: Single ...

The sequence of the structural gene encoding the Legionella pneumophila extracellular zinc metalloprotease has been determined and was found to possess a single large open reading frame (ORF) of 1,629 nucleotides (nt). This ORF was preceded by consensus promoter (TTAACT . . . 17 nt . . . TATAAC) and ribosome-binding (TAAGGAG) sequences. The deduced polypeptide contained a putative signal sequen...

We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%-20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.

Presented here is a draft genome sequence for Staphylococcus agnetis CBMRN 20813338, isolated from a lactating dairy cow with subclinical mastitis. The genome is approximately 2,416 kb and has 35.79% G+C content. Analysis of the deduced open reading frame (ORF) set identified candidate virulence attributes in addition to potential molecular targets for species identification.

We report the draft genome sequence of goose dicistrovirus assembled from the filtered feces of a Canadian goose from South Lake Union in Seattle, Washington. The 9.1-kb dicistronic RNA virus falls within the family Dicistroviridae; however, it shares <33% translated amino acid sequence within the nonstructural open reading frame (ORF) from aparavirus or cripavirus.

We report the genome sequence of a novel iflavirus recovered from the transcriptome of a colony of the brown marmorated stink bug, Halyomorpha halys. The genome is 9,263 nucleotides (nt) and contains an open reading frame (ORF) encoding a 3,017-amino-acid polyprotein.

We report the characterization of human papillomavirus (HPV) subtype 72b of the genus Alphapapillomavirus isolated from an oral rinse sample of a healthy woman. The HPV72b L1 open reading frame (ORF) was 90.2% identical to that of HPV72, indicating a subtype close to the border of a novel HPV type.

The nucleotide sequence of a 2,146 bp portion of the Anacystis nidulans (Synechococcus PCC6301) genome has been determined. This region contains an open reading frame (ORF) of 392 codons, whose predicted protein sequence shows partial homology to those of E. coli phoM and envZ. Hence ORF392 is suggested to be a sensory kinase gene in cyanobacteria.

Polyoxins consist of 14 structurally variable components which differentiate at three branch sites of the carbon skeleton. Open reading frame (ORF) SAV_4805 of Streptomyces avermitilis, showing similarity to thymine-7-hydroxylase, was proved to enhance the diversity of polyoxins at the C-5 site of the 1-(5'-amino-5'-deoxy-β-d-allofuranuronosyl) pyrimidine moiety.

Abbreviations: DMS: Dimethyl Sulphate; ds: Double-Strand; HCV: Hepatitis C Virus; NMIA: N-Methylisatoic Anhydride; NS: Nonstructural; ORF: Open Reading Frame; RT-qPCR: Reverse Transcription-Quantitative Polymerase Chain Reaction; SD: Standard Deviation; SEM: Standard Error of the Mean; SL: Stem-Loop; ss: SingleStrand; SHAPE: Selective 2'-hydroxyl Acylation Analyzed by Primer Extension; UTR: Unt...