There are two optical processes that control the retinal image sampled by the photoreceptor array: aberrations of the ocular optics and cone directionality (Stiles-Crawford effect). The shape of wavefront aberration and Stiles-Crawford functions are known to vary markedly across subjects. In this study we investigate in twelve subjects the symmetry between right and left eyes of wavefront aberr...

PURPOSE To evaluate the impact of disposable soft contact lenses upon visual performance by comparing the optical aberrations of myopic eyes with and without the use of contact lenses. METHOD The optical aberrations of a set of 18 eyes were measured by a laser ray tracing system, an objective measurement method, enabling comparison of the findings of the naked eye, the eye plus a test lens, a...

Two-photon fluorescence microscopy provides a powerful tool for deep tissue imaging. However, optical aberrations from illumination beam path limit imaging depth and resolution. Adaptive Optics (AO) is found to be useful to compensate for optical aberrations and improve image resolution and contrast from two-photon excitation. We have developed an AO system relying on a MEMS Deformable Mirror (...

Current technology allows optical recording of brain activity in awake behaving mice through the use of miniature head-mounted microscopes. These images require processing, as they are unevenly illuminated, contain framewide shifts, and do not readily reveal individual neurons. After statistical methods yield a set of images containing putative individual neuron shapes and locations, a human mu...

Optical aberration effects up to the second moment of Gaussian laser speckle are theoretically investigated for both partially and fully developed speckle. In the development, a plane-wave illuminated diffuser generates a phase-perturbed random field in the object plane that creates speckle in the image plane. Theoretical derivations show that image field statistics are generally non-circular G...

Graded Index (GRIN) rod microlenses are increasingly employed in the assembly of optical probes for microendoscopy applications. Confocal, two-photon and optical coherence tomography (OCT) based on GRIN optical probes permit in-vivo imaging with penetration depths into tissue up to the centimeter range. However, insertion of the probe can be complicated by the need of several alignment and focu...