نتایج جستجو برای: optimized expression
تعداد نتایج: 960581 فیلتر نتایج به سال:
conclusions reteplase was expressed in e. coli top 10 after activation of pbad/giiia promoter region by arabinose and optimized. results the obtained recombinant plasmid was sequenced to confirm the presence and correct framing of reteplase gene regarding the expression of reteplase. maximum production of this enzyme was obtained under the following condition: 0.0002% l-arabinose at 37°c for 2 ...
Article history: Received 12 November 2014 Received in revised form 26 December 2014 Accepted 29 January 2015 Available online 10 February 2015
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Practical high-quality speech and voice synthesis using fixed frame rate ABS/OLA sinusoidal modeling
This paper describes algorithms developed to apply the Analysis-by-Synthesis/Overlap-Add (ABS/OLA) sinusoidal modeling system to real-time speech and singing voice synthesis. As originally proposed, the ABS/OLA system is limited to unidirectional timescaling, and relies on variable frame length to accomplish time-scale modification. For speech and voice synthesis applications, unidirectional ti...
conclusions the results of this study demonstrated that the tpasp-padre-truncated orf2 gene cassette and the truncated orf2 gene in recombinant plasmids are successfully expressed in eukaryotic cells. the immunogenicity of the two recombinant plasmids with different formulations will be evaluated as a novel dna vaccine in future investigations. results sequence analysis and bioinformatics studi...
over the years, the use of plastics has complicated the problem of disposal of solid wastes. one strategy to reduce plastic waste is the use of biodegradable plastics. a group of these plastics are polyhydroxyalkanoates (phas). to date more than 250 different microorganisms are known to synthesize and accumulate pha. most pseu-domonas strains are able to accumulate mcl-pha. in previous studies,...
Green fluorescent protein (GFP), which was originally isolated from jellyfish, is a widely used tool in biological research, and homologs from other organisms are available. However, researchers must determine which GFP is the most suitable for a specific host. Here, we expressed GFPs from several sources in codon-optimized and non-codon-optimized forms in the yeast Saccharomyces cerevisiae, wh...
Adenovirus vector-based vaccine is a promising approach to protect HIV infection. However, a recent phase IIb clinical trial using the vector did not show its protective efficacy against HIV infection. To improve the vaccine, we explored the transgene protein expression and its immunogenicity using optimized codon usage, promoters and adaptors. We compared protein expression and immunogenicity ...
Fragment C is the C-terminal domain of the heavy chain of tetanus toxin that can promote the immune response against the lethal dose of this toxin. Therefore, this portion can be considered as a candidate vaccine against tetanus infection, which occurs by Clostridium tetani. The present study aimed to compare the expression of tetanus toxin fragment C in Escherichia coli BL21 (DE3) pLysS cells...
The genetic code is universal, but recombinant protein expression in heterologous systems is often hampered by divergent codon usage. Here, we demonstrate that reprogramming by standardized multi-parameter gene optimization software and de novo gene synthesis is a suitable general strategy to improve heterologous protein expression. This study compares expression levels of 94 full-length human ...
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