نتایج جستجو برای: optimized expression

تعداد نتایج: 960581  

Journal: :jundishapur journal of natural pharmaceutical products 0
fatemeh shafiee department of pharmaceutical biotechnology, school of pharmacy, isfahan university of medical sciences, isfahan, ir iran fatemeh moazen department of pharmaceutical biotechnology, school of pharmacy, isfahan university of medical sciences, isfahan, ir iran mahammad rabbani department of pharmaceutical biotechnology, school of pharmacy, isfahan university of medical sciences, isfahan, ir iran hamid mir mohammad sadeghi department of pharmaceutical biotechnology, school of pharmacy, isfahan university of medical sciences, isfahan, ir iran; department of pharmaceutical biotechnology, isfahan pharmaceutical sciences research centre, school of pharmacy isfahan university of medical sciences, isfahan, ir iran. tel: +98-3117922622, fax: +98-3116680011

conclusions reteplase was expressed in e. coli top 10 after activation of pbad/giiia promoter region by arabinose and optimized. results the obtained recombinant plasmid was sequenced to confirm the presence and correct framing of reteplase gene regarding the expression of reteplase. maximum production of this enzyme was obtained under the following condition: 0.0002% l-arabinose at 37°c for 2 ...

2015
Jijin Godwin

Article history: Received 12 November 2014 Received in revised form 26 December 2014 Accepted 29 January 2015 Available online 10 February 2015

2003
Maoning Wang Michel Barbeau

...................................................................................................................... III Acknowledgement ............................................................................................................ IV Table of

1998
E. Bryan George

This paper describes algorithms developed to apply the Analysis-by-Synthesis/Overlap-Add (ABS/OLA) sinusoidal modeling system to real-time speech and singing voice synthesis. As originally proposed, the ABS/OLA system is limited to unidirectional timescaling, and relies on variable frame length to accomplish time-scale modification. For speech and voice synthesis applications, unidirectional ti...

Journal: :jundishapur journal of microbiology 0
fatemeh farshadpour department of microbiology and parasitology, school of medicine, bushehr university of medical sciences, bushehr, ir iran; health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran manoochehr makvandi health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran reza taherkhani department of microbiology and parasitology, school of medicine, bushehr university of medical sciences, bushehr, ir iran; persian gulf tropical medicine research center, bushehr university of medical sciences, bushehr, ir iran; department of microbiology, school of medicine, bushehr university of medical sciences, moallem st., bushehr, ir iran. tel: +98-9171712653, fax: +98-77125220814

conclusions the results of this study demonstrated that the tpasp-padre-truncated orf2 gene cassette and the truncated orf2 gene in recombinant plasmids are successfully expressed in eukaryotic cells. the immunogenicity of the two recombinant plasmids with different formulations will be evaluated as a novel dna vaccine in future investigations. results sequence analysis and bioinformatics studi...

Journal: :avicenna journal of medical biotechnology 0

over the years, the use of plastics has complicated the problem of disposal of solid wastes. one strategy to reduce plastic waste is the use of biodegradable plastics. a group of these plastics are polyhydroxyalkanoates (phas). to date more than 250 different microorganisms are known to synthesize and accumulate pha. most pseu-domonas strains are able to accumulate mcl-pha. in previous studies,...

2016
Misato Kaishima Jun Ishii Toshihide Matsuno Nobuo Fukuda Akihiko Kondo

Green fluorescent protein (GFP), which was originally isolated from jellyfish, is a widely used tool in biological research, and homologs from other organisms are available. However, researchers must determine which GFP is the most suitable for a specific host. Here, we expressed GFPs from several sources in codon-optimized and non-codon-optimized forms in the yeast Saccharomyces cerevisiae, wh...

2012
Masaki Shoji Shinji Yoshizaki Hiroyuki Mizuguchi Kenji Okuda Masaru Shimada

Adenovirus vector-based vaccine is a promising approach to protect HIV infection. However, a recent phase IIb clinical trial using the vector did not show its protective efficacy against HIV infection. To improve the vaccine, we explored the transgene protein expression and its immunogenicity using optimized codon usage, promoters and adaptors. We compared protein expression and immunogenicity ...

K. Aghaiypour, R. Teymourpour

Fragment C is the C-terminal domain of the heavy chain of tetanus toxin that can promote the immune response against the lethal dose of this toxin. Therefore, this portion can be considered as a candidate vaccine against tetanus infection, which occurs by Clostridium tetani. The present study aimed to compare the expression of tetanus toxin fragment C in Escherichia coli  BL21 (DE3) pLysS cells...

2010
Barbara Maertens Anne Spriestersbach Uritza von Groll Udo Roth Jan Kubicek Michael Gerrits Marcus Graf Michael Liss Daniela Daubert Ralf Wagner Frank Schäfer

The genetic code is universal, but recombinant protein expression in heterologous systems is often hampered by divergent codon usage. Here, we demonstrate that reprogramming by standardized multi-parameter gene optimization software and de novo gene synthesis is a suitable general strategy to improve heterologous protein expression. This study compares expression levels of 94 full-length human ...

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