AMT1-3 encodes the high affinity NH₄⁺ transporter in rice roots and is predominantly expressed under nitrogen starvation. In order to evaluate the effect of AMT1-3 gene on rice growth, nitrogen absorption and metabolism, we generated AMT1-3-overexpressing plants and analyzed the growth phenotype, yield, carbon and nitrogen metabolic status, and gene expression profiles. Although AMT1-3 mRNA acc...

Abiotic stresses such as salinity and drought impose a severe constraint on global food production, posing serious challenge in agriculture. Stacking beneficial genes transgenic crops will likely improve crop yield under abiotic stress conditions. Previous studies showed that individually overexpressing the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1, protein phosphatase 2 A catalytic sub...

Abstract Tomato production is influenced by shoot branching, which controlled different hormones. Here we produced tomato plants overexpressing the cytokinin-deactivating gene CYTOKININ OXYDASE 2 ( CKX2 ). -overexpressing (CKX2-OE) showed an excessive growth of axillary shoots, opposite phenotype expected for with reduced cytokinin content, as evidenced LC-MS analysis and ARR5-GUS staining. The...

MiR-203 was identified as a hub of potential regulatory miRNA network in central nervous system. Overexpressing miR-203 the frontal cortex C57BL/6J wild type mouse induced neurodegeneration by increasing apoptotic pathway and neuron death. AFF4, transcription factor, new bona fida protein target CNS. The miRNA:mRNA interaction AFF4 verified using Dural-luciferase assay. Down-regulated expressio...

backgoround: plant diseases, caused by a wide range of phytopathogenic fungi, could be managed using of trichoderma sp, as a biocontrol agent. cell wall degrading enzymes like chitinase from t. harzianum are important means for fungal pathogen inhibition. overexpression of these chitinase enzymes can improve the antagonistic potential of trichoderma sp. strains. objectives: this study aimed to ...

Liver damage caused by radiotherapy is associated with a high mortality rate, but no established treatment exists. Adipose-derived mesenchymal stem cells (ADSCs) are capable of migration to injured tissue sites, where they aid in the repair of the damage. Hepatocyte growth factor (HGF) is critical for damage repair due to its anti-apoptotic, anti-fibrotic and cell regeneration-promoting effects...

In this study, we aimed to determine an effective strategy for the synthesis of folate receptor (FR) targeted-nanoparticles (FRNPs). The nanoparticles used as ultrasound contrast agents (UCAs) were composed of a liquid core of perfluorooctyl bromide (PFOB) liposome and a targeted shell chemically conjugated with folic acid (FA) and polyethylene glycol (PEG). This was done in order to avoid reco...

The effects of low root temperature on growth and root cell water transport were compared between wild-type Arabidopsis (Arabidopsis thaliana) and plants overexpressing plasma membrane intrinsic protein 1;4 (PIP1;4) and PIP2;5. Descending root temperature from 25°C to 10°C quickly reduced cell hydraulic conductivity (L(p)) in wild-type plants but did not affect L(p) in plants overexpressing PIP...

OBJECTIVES A possible mechanism for HIV therapy failure is the efflux of HIV drugs from viral target cells or certain body compartments by ATP-binding cassette (ABC) transporters, allowing ongoing viral replication. Here, we investigated the interaction between protease inhibitors (PIs) and ABC transporters. METHODS To explore the potential blocking capacity of PIs, we exposed cells overexpre...

PURPOSE To identify genes whose depletion is detrimental to Pim1-overexpressing prostate cancer cells and to validate this finding in vitro and in vivo. EXPERIMENTAL DESIGN RNAi screening was used to identify genes whose depletion is detrimental to Pim1-overexpressing cells. Our finding was validated using shRNA or PLK1-specific inhibitor BI 2536. Xenograft studies were performed using both P...