Introduction Activation of the Reperfusion Injury SalvageKinase (RISK) pathway, which incorporates phosphatidyli-nositol-3-OH kinase (PI3K)-Akt/protein kinase B (PKB) andp44/42 mitogen-activated protein kinase (MAPK), underliesprotection against ischemia–reperfusion (I/R) injury. Thetemporal nature of the activation of these RISK pathwaycomponents during reperfusion ...

The purpose of this study was to determine the involvement of the p42/p44 mitogen-activated protein kinase (MAPK) pathway and induction of p21(waf1/cip1) in the antiproliferative effects of nitric oxide (NO) on rat aortic smooth muscle cells (RASMC). NO, like alpha-difluoromethylornithine (DFMO), interferes with cell proliferation by inhibiting ornithine decarboxylase (ODC) and, therefore, poly...

In collaborative design and distributed manufacturing, the need to co-develop parts by designers at different geographical locations often arises. For designing a promising product, there is always a need for collaboration among the design, marketing, financial and procurement departments, and the top management. Global manufacturing makes it difficult to frequently gather all the departments i...

The local renin-angiotensin system is present in the pituitary. We investigated the effects of angiotensins on GH3 lactosomatotroph cells proliferation in vitro and the involvement of p44/42 and p38 MAPK inhibitors in the growth-regulatory effects of angiotensins. Materials and Methods. Cell viability using the Mosmann method and proliferation by the measurement of BrdU incorporation during DNA...

Protein kinase C (PKC)-alpha, -betaI, and -delta are known to be involved in the lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages. The role of mitogen-activated protein kinases (MAPK) p44/42 and p38 in the LPS effect was studied further. LPS-mediated NO release and the inducible form of NO synthase expression were inhibited by the p38 inhibitor, SB 203580, ...

The Rift Valley fever virus (RVFV) is the causative agent of fatal hemorrhagic fever in humans and acute hepatitis in ruminants. We found that infection by RVFV leads to a rapid and drastic suppression of host cellular RNA synthesis that parallels a decrease of the TFIIH transcription factor cellular concentration. Using yeast two hybrid system, recombinant technology, and confocal microscopy, ...

Aliaga, José Cristobal, Claude Deschênes, JeanFrançois Beaulieu, Ezéquiel L. Calvo, and Nathalie Rivard. Requirement of the MAP kinase cascade for cell cycle progression and differentiation of human intestinal cells. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G631–G641, 1999.—The intracellular signaling pathways responsible for cell cycle arrest and establishment of differentiated c...

Background—Although Janus kinase (JAK)–mediated Tyr phosphorylation of signal transducers and activators of transcription (STAT) 1 and 3 is essential for the upregulation of cyclooxygenase-2 (COX-2) and the cardioprotection of late preconditioning (PC), the role of Ser phosphorylation of STAT1 and STAT3 in late PC and the upstream signaling mechanisms responsible for mediating Ser phosphorylati...

Clinical and animal studies have shown that treatment with angiotensin-converting enzyme (ACE) inhibitors or angiotensin II (Ang II) receptor antagonists slows the progression of nephropathy in diabetes, indicating that Ang II plays an important role in its development. We have reported previously that insulin inhibits the stimulatory effect of high glucose levels on angiotensinogen (ANG) gene ...