Plasmid pP51 of Pseudomonas sp. strain P51 contains two gene clusters encoding the degradation of chlorinated benzenes, tcbAB and tcbCDEF. A regulatory gene, tcbR, was located upstream and divergently transcribed from the chlorocatechol oxidative gene cluster tcbCDEF. The tcbR gene was characterized by DNA sequencing and expression studies with Escherichia coli and pET8c and appeared to encode ...

This paper attempts to portray a synthesis of what has been learned in the past 10 years with regard to the transition process. It contrasts the mainstream “Washington consensus” view of transition with the “evolutionary institutionalist” perspective. It argues that the latter gives a more adequate and complete picture both of the transition processes and of economic systems and is of better he...

We previously identified a rare mutation in human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT), I132M, which confers high-level resistance to the nonnucleoside RT inhibitors (NNRTIs) nevirapine and delavirdine. In this study, we have further characterized the role of this mutation in viral replication capacity and in resistance to other RT inhibitors. Surprisingly, our data ...

In order to study the inferences of structure for mechanism, the collective motions of the retroviral reverse transcriptase HIV-1 RT (RT) are examined using the Gaussian network model (GNM) of proteins. This model is particularly suitable for elucidating the global dynamic characteristics of large proteins such as the presently investigated heterodimeric RT comprising a total of 982 residues. L...

Background Therapeutic vaccination against prostate cancer (CaP) remains marginally effective. A reason for failure may stem from the fact that vaccine cells are usually cultured in ordinary air. Solid tumours including CaP contain regions with oxygen deficiency (hypoxia) secondary to the lack of blood supply to the growing tumour nodules, which may lead to changes in expression of cancer-assoc...