PapD is a periplasmic chaperone essential for P pilus formation in pyelonephritic strains of E. coli. It is composed of two domains, each of which contains a tryptophan residue (Trp-36 and Trp-128, in the N- and C-terminal domains, respectively). To explore the role of domain-domain interactions during folding, the protein was labeled with 6-fluorotryptophan for use in (19)F-NMR experiments. (1...

P pili are extracellular appendages responsible for the targeting of uropathogenic Escherichia coli to the kidney. They are assembled by the chaperone-usher (CU) pathway of pilus biogenesis involving two proteins, the periplasmic chaperone PapD and the outer membrane assembly platform, PapC. Many aspects of the structural biology of the Pap CU pathway have been elucidated, except for the C-term...

Transcription of the genomic region involved in gas vesicle formation in Halobacterium salinarum (p-vac) and Haloferax mediterranei (mc-vac) is driven by two divergent promoters, P(A) and P(D), separated by only 35 nt. Both promoters are activated by the transcription activator GvpE which in the case of P(mcA) requires a 20-nt sequence (UAS) consisting of two conserved 8-nt sequence portions lo...

Submitted for the MAR13 Meeting of The American Physical Society Topological phase transition in hexagonal boron-nitride bilayers modulated by gate voltage1 GUOJUN JIN, XUECHAO ZHAI, Nanjing University — We study the gate-voltage modulated electronic properties of hexagonal boron-nitride bilayers with two different stacking structures in the presence of intrinsic and Rashba spin-orbit interacti...

*Corresponding author e-mail: [email protected] **This work was financial supported from China 863 HI-TECH R and D Program (No. 2011AA100802), National Natural Science Foundation of China (Grant No. 61271384 and 1275155), the 111 Project (B12018) and PAPD of Jiangsu Higher Education Institutions and sponsored by Qing Lan Project, 2014-2016. A b s t r a c t. Moisture content uniformity is one ...

The properties of the pediocin AcH precursor, prepediocin AcH, have been studied to gain insight into how producer cells may protect themselves from the activity of intracellular prebacteriocins. The native 62-amino-acid precursor and the 44-amino-acid mature species were expressed in Escherichia coli host strains that lack the leader peptide processing enzyme, PapD. Both forms inhibited the gr...

one of the most important and elusive diseases of grapevine is esca disease. esca is a complex disease that comprises a combination of symptoms caused by various fungi including growth retardation, decrease in productivity and decline of the plant and usually affecting adult plants (8 to 10 years and older). the purpose of this research was to study the genetic variations of fungal agents assoc...

OBJECTIVE To assess the effect of cilazapril treatment on several hemodynamic parameters during handgrip maneuvers in patients with congestive heart failure. Cilazapril, an ACE inhibitor with high affinity, has been shown to be highly effective against a variety of vascular disorders. The effectiveness of isometric handgrip exercise on changes of cardiovascular hemodynamic parameters before and...

The periplasmic molecular chaperone Caf1M of Yersinia pestis is a typical representative of a subfamily of specific chaperones involved in assembly of surface adhesins with a very simple structure. One characteristic feature of this Caf1M-like subfamily is possession of an extended, variable sequence (termed FGL) between the F1 and subunit binding G1 beta-strands. In contrast, FGS subfamily mem...