Determination of parentage is fundamental to the study of biology and to applications such as the identification of pedigrees. Limitations to studies of parentage have stemmed from the use of an insufficient number of hypervariable loci and mismatches of alleles that can be caused by mutation or by laboratory error and that can generate false exclusions. Furthermore, most studies of parentage h...

To develop an efficient DNA typing system for Chinese Holstein cattle, 17 microsatellites, which were amplified in four fluorescent multiplex reactions and genotyped by two capillary electrophoresis injections, were evaluated for parentage verification and identity test. These markers were highly polymorphic with a mean of 8.35 alleles per locus and an average expected heterozygosity of 0.711 i...

AIM To report on the successful use of Laser Capture Microdissection (LCM) as a tool for isolation of human chorionic villi from admixed maternal tissue. Subsequent DNA isolation for forensic short tandem repeat (STR) analysis for parentage testing was performed in two cases of alleged sexual assault of female victims. We also performed validation of the LCM instrument platform, using archival ...

The aim of the present study was the conducting of comparative analysis of allele pool of Ukrainian population of Thoroughbred horses and the populations from England, USA, Russia and South Korea using microsatellite loci of DNA on the basis of our own researches and literary sources. Comparative analysis of allele pool of Thoroughbred populations from different countries was conducted using 6 ...

A study was aimed to evaluate a set of polymorphic microsatellite markers for their efficiency in testing parentage Jersey crossbred cattle available Tamil Nadu. The objective validation based on the criteria such as polymorphism information content, various genetic diversity parameters and ability exclude wrong parent. total 21 were included verify 24 trios (comprising dams, progenies 2 sires)...

A civil paternity investigation involving the parents of deceased alleged father in order to establish a family relationship is presented. On basis 23 autosomal short tandem repeat (aSTR) genotyping results, conclusive proof was not achieved, as probability (W) calculated 0.99988. Additional genetic data 17 classical and non-classical human leukocyte alleles (HLA) typing by next-generation sequ...

the aim of the present study was the conducting of comparative analysis of allele pool of ukrainian population of thoroughbred horses and the populations from england, usa, russia and south korea using microsatellite loci of dna on the basis of our own researches and literary sources. comparative analysis of allele pool of thoroughbred populations from different countries was conducted using 6 ...

DNA microsatellites provide plant ecologists with molecular markers precise enough to assign parentage to seeds and seedlings. This allows the exact distance and trajectory of successful pollen to be traced to characterize pollination patterns. Parentage assignment of established seedlings also allows researchers to accurately determine how far new recruits have traveled from their seed parent....

Likelihood-based parentage inference depends on the distribution of a likelihood-ratio statistic, which, in most cases of interest, cannot be exactly determined, but only approximated by Monte Carlo simulation. We provide importance-sampling algorithms for efficiently approximating very small tail probabilities in the distribution of the likelihood-ratio statistic. These importance-sampling met...