An heterologous expression of Vitreoscilla hemoglobin (VHb) for improving cell growth and recombinant protein production has been successfully demonstrated in various hosts, including Pichia pastoris. Lower temperature cultures can enhance target protein production in some studies of P. pastoris. In this study, the strategy of combining heterologous VHb expression and lower temperature cultures...

OBJECTIVES The purpose of this study was to verify the feasibility of using the glyceraldehyde-3-phosphate dehydrogenase (GAP) promotor based Pichia pastoris expression system to produce tick-borne encephalitis virus (TBEV) virus-like particles (VLPs). METHODS The complementary DNA encoding the TBEV prM signal peptide, prM, and E proteins of TBEV Korean strain (KrM 93) was cloned into the pla...

Alessandro Cresti , Giuseppe Grosso (2) and Giuseppe Pastori Parravicini (3) (1) NEST-INFM, Scuola Normale Superiore, Piazza dei Cavalieri 7, I-56126 Pisa, Italy, and Dipartimento di Fisica E. Fermi, Largo B. Pontecorvo 3, I-56127 Pisa, Italy [email protected] (2) NEST-INFM and Dipartimento di Fisica E. Fermi, Largo B. Pontecorvo 3, I-56127 Pisa, Italy (3) NEST-INFM and Dipartimento di Fisica ...

lipases are versatile biocatalysts with a wide range of application in food, dairy, leather, paper, pharmaceutical and detergent industries.  in this study, mesophilic lipase gene from an indigenous bacillus pumilus f3 that already its gene had been sequenced and  identified, was cloned and expressed in methylotrophic yeast pichia pastoris and codon translation of lipase gene was evaluated in p...

objectives in the present study, we evaluated the antibacterial activity of capsella bursa-pastoris alone and also combined with glycyrrhiza glabra against streptococcus mutans, s. sanguis, actinomyces viscosus, enterococcus faecalis as oral pathogens. conclusions c. bursa-pastoris and its mixture with g. glabra are suggested as appropriate candidates to control dental caries and endodontic inf...

BACKGROUND The methylotrophic yeast, Pichia pastoris, is widely used as a useful experimental tool in protein engineering and production. It is common for proteins expressed in P. pastoris to exhibit N-glycosylation. In recent years, glycosylation studies in P. pastoris have attracted increasing attention from scholars. Rhizopus chinensis lipase (RCL) is one of the most important industrial lip...

BACKGROUND The large-scale production of G-protein coupled receptors (GPCRs) for functional and structural studies remains a challenge. Recent successes have been made in the expression of a range of GPCRs using Pichia pastoris as an expression host. P. pastoris has a number of advantages over other expression systems including ability to post-translationally modify expressed proteins, relative...

A gene (lsd1) encoding dextranase from Lipomyces starkeyi KSM22 has been previously cloned, sequenced, and expressed in Saccharomyces cerevisiae. The gene consisting of 1,824 base pairs and encoding a protein of 608 amino acids was then cloned into and secretively expressed in Pichia pastoris under the control of the AOX1 promoter. The dextranase productivity of the P. pastoris transformant (pP...

−We established a gene expression process to produce a flavor peptide in Pichia pastoris. The octapeptide Lys-Gly-Asp-Glu-Glu-Ser-Leu-Ala was isolated from beef digested by papain, known as “beefy meaty peptide” (BMP). It was considered a savory seasoning in previous literatures. To produce BMP by microorganisms, BMP expression structures encoding tandem repeats of the octapeptide were designed...