Introduction: Beta-carboline alkaloids of P. harmala are shown to have immune-modulatory effects in several studies. Extracts of this plant have significant anti-inflammatory effect via the inhibition of some inflammatory mediators including PGE2 and TNF-α. In postmortem studies, structural alterations to the cerebellum have been recognized, including Purkinje cell loss being re...

Patch-clamp recording techniques have revolutionized understanding of the function and sub-cellular location of ion channels in excitable cells. The cell-attached patch-clamp configuration represents the method of choice to describe the endogenous properties of voltage-activated ion channels in the axonal, somatic and dendritic membrane of neurons, without disturbance of the intracellular milie...

Midbrain dopaminergic neurons (mDA neurons) are essential for the control of diverse motor and cognitive behaviors. However, our understanding of the activity of immature mDA neurons is rudimentary. Rodent mDA neurons migrate and differentiate early in embryonic life and dopaminergic axons enter the striatum and contact striatal neurons a few days before birth, but when these are functional is ...

In this projecting work we propose a mass spectrometric patch-clamp equipment with the capillary performing both a local potential registration at the cell membrane and the analyte suction simultaneously. This paper provides a current literature analysis comparing the possibilities of the novel approach proposed with the known methods, such as scanning patch-clamp, scanning ion conductance micr...

Background: Ketamine is a commonly used intravenous anesthetic which produces dissociation anesthesia, analgesia, and amnesia. The mechanism of ketamine-induced synaptic inhibition in high-level cortical areas is still unknown. We aimed to elucidate the effects of different concentrations of ketamine on the glutamatergic synaptic transmission of the neurons in the primary somatosensory cortex b...

Abstract Patch clamp recording of neurons is a labor-intensive and time-consuming procedure. Here, we demonstrate tool that fully automatically performs electrophysiological recordings in label-free tissue slices. The automation covers the detection cells images, calibration micropipette movement, approach to cell with pipette, formation whole-cell configuration, recording. based on deep learni...

Introduction: The Cannabinoid receptors (CBR) densities are high within the cerebellum. Cannabinoid receptors manipulations have been reported to cause altering the cerebellar functions. harmaline have immune-modulatory effects in several studies. i.e., significant anti-inflammatory effect via the inhibition of prostaglandin E2 (PGE2) and tumor necrosis factor alpha (TNF-α). Endocannabino...