The ATPase Inhibitory Factor 1 (IF1) is an inhibitor of the mitochondrial H+-ATP synthase that regulates the activity of both oxidative phosphorylation (OXPHOS) and cell death. Here, we have developed transgenic Tet-On and Tet-Off mice that express a mutant active form of hIF1 in the hepatocytes to restrain OXPHOS in the liver to investigate the relevance of mitochondrial activity in hepatocarc...

Objective(s): Recent studies revealed that microRNAs (miRNAs) may play crucial roles in the responses and pathologic processes of spinal cord injury (SCI). This study aimed to investigate the effect and the molecular basis of miR-103 on LPS-induced injuries in PC12 cells in vitro and SCI rats in vivo. Materials and Methods: PC12 cells were exposed to LPS to induce cell injuries to mimic the in ...

Objective(s): This study was designed to indicate whether different fractions from Artemisia biennis hydroethanolic extract could provide cytoprotection against oxidative stress and apoptosis induced by doxorubicin (DOX) in rat pheochromocytoma cell line (PC12). Material and Methods:Cell viability was determined by MTT assay. Also, activation of caspase-3 and superoxide dismutase were evaluated...

Objective(s): According to recent studies, valproate shows some protection against oxidative stress (OS) induced by neurotoxins. Current investigation tried to determine the possible ameliorating effects of sodium valproate (SV) against aluminum (Al)-induced cell death, apoptosis, mitochondrial membrane potential (MMP), and OS in PC12 cells.Materials ...

objective: annexin a1 (anxa1) is suggested to have anti-inflammatory function. however, the precise function of anxa1 has remained unclear. in this study, we therefore examined the potency of anxa1 in regulating reactive oxygen species (ros) production and suppressing pro-inflammatory responses in pc12 cells induced by 1-methyl-4-phenylpyridinium (mpp+). materials and methods: in this experimen...

The large dense-core secretory granule is an organelle in neuroendocrine/endocrine cells, where prohormones and proneuropeptides are stored, processed, and secreted in a regulated manner. Here we present evidence that chromogranin A (CgA), one of the most abundant acidic glycoproteins ubiquitously present in neuroendocrine/endocrine cells, regulates dense-core secretory granule biogenesis. Spec...

Background and Aims:In neurodegenerative disorders,oxidative stress mediated by reactive oxygen species is strongly associated with increased neuronal damages which can lead to apoptosis. Pro-apoptotic and anti-apoptotic gene expressions are changed during the cell differentiation that affect cell viability and differentiation. Therefore, this study was conducted to determine the effects of hyd...

objective: the discovery and development of natural products with potent antioxidant properties has been one of the most interesting and promising approaches in the search for treatment of cns injuries. the most significant consequence of the oxidative stress is thought to be the dna modifications, which can become permanent via the formation of mutations and other types of genomic instability ...

Application of nanotechnology in medicinal and biological fields has attracted a great interest in the recent yeras. In this paper the cell membrane leakage induced by iron nanoparticles (Fe-NP) against PC12 cell line which is known as a model of nervous system cell line was investigated by the lactate dehydrogenase (LDH) test. Therefore, PC12 cells were incubated with different concentration o...

Resistance to bacterial speck disease in tomato (Solanum lycopersicum) is activated upon recognition by the host Pto kinase of either one of two sequence-unrelated effector proteins, AvrPto or AvrPtoB, from Pseudomonas syringae pv tomato (Pst). Pto induces Pst immunity by acting in concert with the Prf protein. The recently reported structure of the AvrPto-Pto complex revealed that interaction ...