A Review of Optimization Strategies and the Advantages Disadvantages using Polymerase Chain Reaction with Confronting Two-Pair primers (PCR-CTPP) in Genomics Studies

A Short History of the Polymerase Chain Reaction. In the 1950s, the discovery of PCR is the subject of claim and counterclaim that has yet to be.Methods Mol Biol. A short history of the polymerase chain reaction. Author information: 1Division of Cancer and.A Short History of the Polymerase Chain Reaction. Full Text, Download PDF 103K. And although this does risk overstating the impact of PCR ou...

macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). th...

results our study showed that the sensitivity and specificity of taqman real time pcr was 96% and 95% respectively. these values were 89% and 90, 38% and 100%, 6% and 100% for pcr, culture and microscopy, respectively. conclusions our study showed that sensitivity of taqman real time pcr was higher that pcr, culture and microscopy but specificity of culture and microscopy was more than pcr and ...

Background: Molecular detection has recently been proposed by nucleic acid amplification, known as polymerase chain reaction (PCR). The aim of this study was to compare the diagnostic method of smear and polymerase chain reaction with culture in terms of sensitivity, specificity, positive and negative predictive value in the diagnosis of pulmonary tuberculosis. Methods: In this cross-sectional...

introduction and aims: the role of human papillomavirus (hpv) in the etiology of esophageal squamous cell carcinoma (escc) is not clear. previous studies have found highly variable (from 0% to 67%) prevalence of hpv in escc tumor. however, prevalence of hpv in escc tumor seems to be higher in areas with high incidence of escc , such as china and south africa. iran is one of the areas of the wor...

conclusions the observed results indicated the necessity of good care in abattoir, butcheries and during food distribution, mainly ground beef. also, a zero tolerance policy could be useful to control e. coli o157 in meat products nationwide. results overall, 1.5% of ground beef samples were contaminated with the o157 e. coli strain meanwhile 1% of samples contained the o157:h7 strain and 0.5% ...