The emergence and re-emergence of various viral diseases indicate the need for the development of effective and authentic new diagnostic methods. The diagnostic application of gel-based PCR, general PCR systems, phylogeny, molecular epidemiology, real-time PCR, amplification without thermo-cycling, multiplex PCR, nucleic acid extraction and pipetting robotics, automation and quality control, in...

BACKGROUND Interindividual differences in CYP2D6 activity range from total absence of metabolism of certain drugs to ultrafast metabolism and can produce adverse effects or lack of therapeutic effect under standard therapy. Several mutations have been described in the CYP2D6 gene that abolish CYP2D6 activity. However, four mutations explain the majority of the poor metabolizers. We describe fou...

Vector construction with restriction enzymes (REs) typically involves the ligation of a digested donor fragment (insert) to a reciprocally digested recipient fragment (vector backbone). Creating a suitable cloning plan becomes increasingly difficult for complex strategies requiring repeated insertions such as constructing multiple short hairpin RNA (shRNA) expression vectors for RNA interferenc...

Accurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), the most commonly used detection method for YMV, detected the virus in significantly less leaf sa...

Ultraviolet (UV)-induced cataracts are becoming a major environmental health concern because of the possible decrease in the stratospheric ozone layer. Experiments were designed to isolate gene(s) affected by UV irradiation in rabbit cornea tissues using fluorescent differential display-reverse transcription-polymerase chain reaction (FDDRT-PCR). The epithelial cells were grown in standard medi...

Abstract Objective We investigated the discrepancy between clinical and PCR-based diagnosis of COVID-19. compared results ten patients with mild to severe Respiratory samples from all cases were tested on Roche SARS-CoV-2 (Cobas) assay, Filmarray RP2.1 (bioMereiux) TaqPath™ COVID19 (Thermofisher) PCR assays. Results Laboratory records COVID-19 examined. Initially, respiratory as negative ® assa...

Despite all blood donations being tested routinely for HBsAg as a clinical marker of transmissible HBV, cases post-transfusion hepatitis B virus infection are still reported because molecular studies using Polymerase Chain Reaction (PCR) not available Transfusion Transmissible Infection (TTIs) testing. In this study, we sought to use the PCR technique re-screen donated that had already been pro...

Comments Re a) After individual genetic counseling with regard to the risk of miscarriage caused by the invasive procedure and the risk of fetal chromosomal abnormality, every pregnant woman/couple decides whether an amniocentesis should be performed. From a strictly medical point of view, it is desirable that the risk of chromosomal abnormality be at least as high as that of miscarriage caused...