background human papillomavirus (hpv) is associated with various benign and malignant lesions including genital condyloma and anogenital cancer. the presence of hpv-dna was studied in archival biopsies of high- and intermediate-risk lesions for cervical carcinoma in women referred to mirza hospital in tehran. patients and methods totally, 105 iranian archived biopsies collected during february ...

A variety of factors that influence real-time reverse transcriptase (RT)-PCR results were examined. Realtime reaction volumes can be decreased from the manufacturers suggested volumes (50μl) to as little as 5μl. Errors introduced through volume handlings can be significantly mitigated through the use of electronic pipettes. Random hexamers are compared against the use of a specific reverse tran...

In this project, real time polymerase chain reaction (PCR) was utilized to study the mechanism of PCR inhibition through examination of the effect of amplicon length, melting temperature, and sequence. Specifically designed primers with three different amplicon lengths and three different melting temperatures were used to target a single homozygous allele in the HUMTH01 locus. The effect on amp...

Polymerase chain reaction (PCR) is largely used in molecular biology for increasing the copy number of a specific DNA fragment. The succession of 20 replication cycles makes it possible to multiply the quantity of the fragment of interest by a factor of 1 million. The PCR technique has revolutionized genomics research. Several quantification methodologies are available to determine the DNA repl...

Real Time Polymerase Chain Reaction also known as quantitative PCR (q-PCR) is a widely used tool in microbial ecology to quantify gene abundances of taxonomic and functional groups in environmental samples. Used in combination with a reverse transcriptase reaction (RT-q-PCR), it can also be employed to quantify gene transcripts. q-PCR makes use of highly sensitive fluorescent detection chemistr...

Background and Aim: QX-like infectious bronchitis virus (IBV) is a highly avian coronavirus that causes respiratory kidney disease. It linked to increased mortality loss of performance in infected chickens worldwide, including Thailand. Thus, simple rapid diagnostic method for the diagnosis IBV needed. This study aimed develop single-step multiplex reverse transcription-polymerase chain reactio...

In this paper, we generalize a previously-described model of the error-prone polymerase chain reaction (PCR) reaction to conditions of arbitrarily variable amplification efficiency and initial population size. Generalisation of the model to these conditions improves the correspondence to observed and expected behaviours of PCR, and restricts the extent to which the model may explore sequence sp...

A Review of Optimization Strategies and the Advantages Disadvantages using Polymerase Chain Reaction with Confronting Two-Pair primers (PCR-CTPP) in Genomics Studies