Quantitative qPCR is a routinely used method for the accurate quantification of nucleic acids. Yet it may generate erroneous results if the amplification process is obscured by inhibition or generation of aberrant side-products such as primer dimers. Several methods have been established to control for pre-processing performance that rely on the introduction of a co-amplified reference sequence...

Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for ...

The basis for the identification of SARS-CoV-2 cases in handling COVID-19 pandemic is Reverse Transcriptasi Polymerase (RT-PCR) and specific rapid antigen test. RT-PCR test takes a long time expensive to get results, while simple fast alternative getting detection results. method used descriptive observational form literature review by reviewing existing literature. Several screening confirmato...

The incorporation of a commercially available coprecipitant into the AMPLICOR enterovirus PCR test specimen preparation enhanced the sensitivity and reproducibility of this assay. Fifty-five previously tested archived cerebrospinal fluids (CSF) specimens were tested in a blind study in duplicate with and without Pellet Paint coprecipitant (Novagen, Inc., Madison, Wis.). Of these specimens, 26 h...

375 A 1-year-old castrated male domestic shorthaired cat was presented for evaluation of a 10-day history of lethargy, anorexia, decreased water consumption, and apparent discomfort. A complete blood count and serum chemistry profile revealed hypoalbuminemia, leukocytosis, and neutrophilia. The results of a combined ELISA test for feline leukemia virus and feline immunodeficiency virus were neg...

method correlated well with the other two compared hs-CRP methods in the range of 0.2–100 mg/L. At concentrations 100 mg/L, however, poor correlations were found between all compared methods. Because we are convinced that for the clinical laboratory it is most practical to use one CRP method for the complete measuring range, further investigation to improve agreement among the different methods...

BACKGROUND Tinea cruris is the second most common dermatophytosis in the world and the most common in Indonesia. The conventional laboratory tests for dermatophyte infection are slow and less specific. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with the addition of enzyme after amplification, therefore enabling for more specific results. AIM ...