This study presents a smart technological framework to efficiently remove azithromycin from natural soil resources using bioremediation techniques. The consists of several modules, each with different models such as Penicillium Simplicissimum (PS) bioactivity, soft computing models, statistical optimisation, Machine Learning (ML) algorithms, and Decision Tree (DT) control system based on Remova...

Catalases, peroxidases, and catalase-peroxidases are important enzymes to cope with reactive oxygen species in pro- and eukaryotic cells. In the filamentous fungus Aspergillus nidulans three monofunctional catalases have been described, and a fourth catalase activity was observed in native polyacrylamide gels. The latter activity is probably due to the bifunctional enzyme catalase-peroxidase, w...

In a natural ecosystem, endophytic fungi in the internal tissues of plants help to improve growth host and decrease negative effects biotic abiotic stresses without having adverse effects. Japan, Vicia villosa (hairy vetch), legume plant with high capacity fix nitrogen, is usually used as cover crop before soybeans enhance fertility structure soil. This study aimed isolate associated different ...

A large number of microorganisms are capable of degrading cellulose, only a few of these microorganisms produce significant quantities of enzymes capable of completely hydrolysing cellulose. Fungi are the main cellulase-producing microorganisms. In this purposed study, seventeen fungal species belonging to three genera i.e. Trichoderm, Aspergillus and Penicillium were isolated from different so...

Eugenol oxidase (EUGO) from Rhodococcus jostii RHA1 had previously been shown to convert only a limited set of phenolic compounds. In this study, we have explored the biocatalytic potential of this flavoprotein oxidase, resulting in a broadened substrate scope and a deeper insight into its structural properties. In addition to the oxidation of vanillyl alcohol and the hydroxylation of eugenol, ...

<p>Rimpang jahe merah digunakan sebagai tumbuhan obat tradisional dan diketahui mengandung senyawa-senyawa berkhasiat. Dekade ini eksplorasi mikroorganisme yang bersimbiosis dengan mendapat perhatian besar guna mencari alternatif sumber senyawa berkhasiat obat. Penelitian bertujuan memperoleh informasi profil kimia ekstrak <em>Penicillium simplicissimum</em>, jamur endofit dii...

Enzyme extraction from solid matrix is as important step in solid-state fermentation to obtain soluble enzymes for further immobilization and application in biocatalysis. A method for the recovery of a pool of lipases from Penicillium simplicissimum produced by solid-state fermentation was developed. For lipase recovery different extraction solution was used and phosphate buffer containing Twee...

Vanillyl-alcohol oxidase (VAO) from Penicillium simplicissimum is an inducible flavoprotein involved in the biodegradation of lignin-derived aromatic compounds. The enzyme is the prototype of a newly recognized family of structurally related oxidoreductases, whose members share a conserved FAD-binding domain. The flavin cofactor in VAO is covalently linked to His422 of the cap domain. This cova...