The present study was aimed to examine the association of bovine follicular stimulating hormone gene polymorphism with sperm quality traits including sperm volume (SV), sperm concentration (SPCO), total sperm (TS), fresh sperm motility (FSM), total fresh motile sperm (TFMS), post thaw sperm motility (PTSM), total post thaw motile sperm (TPTMS), number of produced payout (NPP), number of fresh m...

To illustrate the influence of kallikrein on the hypoosmotic swelling test, 84 human semen samples were classified into 4 groups: normospermia (n = 34, group A), oligospermia (n = 18, group B), asthenospermia (n = 8, group C), and oligoasthenospermia (n = 24, group D). Two aliquots of 0.45 ml were prepared from each specimen in all the groups: one was incubated for 3 hr in a glass tube containi...

BACKGROUND Isolation of sperm suitable for ICSI from fresh or frozen-thawed testicular sperm extraction (TESE) can be facilitated by mechanical or enzymatic processing of the samples. METHODS A retrospective multicentre study was initiated to compare these two approaches. Eleven German centres provided data on their TESE cycles performed during the period 1996/1997. Quality of retrieved sperm...

BACKGROUND Sperm screening is an essential step in in vitro fertilization (IVF) procedures. The swim-up method, an assay for sperm motility, is used clinically to select the ideal sperm for subsequent manipulation. However, additional parameters, including acrosome reaction capability, chemotaxis, and thermotaxis, are also important indicators of mammalian sperm health. To monitor both sperm mo...

The objective of this study was to compare (i) the intracytoplasmic sperm injection outcomes among groups with different total motile sperm count ranges, (ii) the intracytoplasmic sperm injection outcomes between groups with normal and abnormal total motile sperm count, and (iii) the predictive values of WHO 2010 cut-off values and pre-wash total motile sperm count for the intracytoplasmic sper...

A modified chemotaxis chamber was used to evaluate cellulose acetate/nitrate filters for the measurement of dog sperm motility. The distance travelled into the filters was affected by filter pore size, incubation time and sperm concentration. After storage of spermatozoa at 37 degrees C the distance penetrated into the filters reflected the deterioration of the sample, but was not as sensitive ...

The presence of a complex population of mRNAs in human mature spermatozoa is well documented; among them, transcripts of aromatase and ERs (oestrogen receptors) have been described but their significance is not clear. Therefore, to clarify the role of this complex population of mRNAs in human ejaculated sperm, we have isolated on discontinuous density gradients two main fractions from the same ...

The hypothesis was tested that frozen bovine oviduct epithelial cells (BOEC) could provide a physiologic and standardized system for studying motility and acrosomal changes of fresh and frozen-thawed sperm. The BOEC were collected by lavage from cows and monolayers prepared from both fresh and frozen-thawed cells. Fresh and frozen semen processed from five bulls was extended in a modified Tyrod...

Chronic bacterial prostatitis (CBP) is caused by bacterial infection and maintains a condition of lower urinary tract infection. It may be a cause of male infertility. However, studies showed inconsistent results regarding the effect of CBP on several parameters of semen. Hence, we conducted a meta-analysis to examine the effect of CBP on basic semen parameters. A systematic review was conducte...

In this study, age-dependent changes of sperm quality parameters in Rutilus frisii were investigated. Also, fertilization capacity of breeders was tested using two age groups of males (3 and 4 year old breeders) and one female group (i.e. 4 year old breeders). For fertilization trials, the breeders were crossed randomly among age classes. Between the two age groups, spermatological parameters d...