We have investigated the periodic acid Schiff (PAS) Coomassie staining ratio of the human factor VIII/von Willebrand factor (fVIII/vWf) protein. The PAS-Coomassie staining ratio is consistent over 8 days. The PAS-Coomassie ratio of fVIII/vWf protein purified from different starting materials does not appear to be significantly different. The PAS stain can detect as little as 300 ng of carbohydr...

The lymphocyte glycogen content has been measured semi-quantitatively by staining peripheral blood films by the periodic-acid-Schiff (P.A.S.) technique. The high values reported in chronic lymphatic leukaemia, lymphosarcoma, Hodgkin's disease, and infectious mononucleosis have been confirmed. High values have also been found in cases with chronic suppuration, ulcerative colitis, myelomatosis, a...

Pulmonary alveolar proteinosis (PAP) is a rare lung disease where periodic acid Schiff (PAS)-positive eosinophilic material accumulates in the alveoli of the lungs. Here we describe two cases of young males who presented with dynpnoea and weight loss. The HRCT scan of the chest in both cases showed the typical "crazy-paving" pattern and lung biopsies confirmed the diagnosis of PAP. They showed ...

By GEORGE B . WISLOCKI, M.D., JACK J. RHEINGOLD, M.D., AND EDWARD \V. DEMPSEY, PH.D. T HE PERIODIC acid-Schiff method stains glycogen, mucus, reticulum and basement membranes, some kinds ofelastic tissue, fibrin, and various substances ofquite unknown composition (McManus’; Lillie et al.’; Wislocki and Dempsey3). Glycogen may be differentiated from these other substances by the fact that it is ...

Idoxuridine-treated McCoy cells grown as monolayers in 96 well microplates provide a convenient method for the isolation of Chlamydia trachomatis. Staining of infected monolayers with periodic acid-Schiff reagent (PAS) allows easy recognition of C trachomatis inclusions without the need for dark-ground microscopy. By this method 384 clinical specimens can be examined concurrently. It is suffici...

Figure 1. Upper left, Photograph showing skin lesions on arm and shoulder of patient. Upper right, CT scan of neck revealing multiple enlarged lymph nodes. Lower left, Detail of a skin ulcer on forehead of patient. Lower right, Histologic examination of skin biopsy specimen showing perivascular infiltrates of neutrophil granulocytes and ovoid yeasts (diameter, 15 mm) and a thick cell wall (peri...

Preliminary to a study of benign appendageal tumors of the skin, the results of which will be published separately, the following study of normal apocrine and eccrine glands was undertaken. The use of the periodic acid-Schiff (PAS) and Alcian Blue (AB) stains separately on tissue sections and the clarification of the two color reactions of the PAS stain by Alcian Blue (1) has lead to a better h...

PURPOSE/METHODS To report a rare case of a tumor in a conjunctival location, a giant cell collagenoma. Tissue was stained with hematoxylin-eosin, periodic acid-Schiff, and Masson's trichromic stain and studied by immunohistochemistry. RESULTS/CONCLUSION The clinical and histopathologic features of conjunctival giant cell collagenoma are similar to characteristics of the same tumor occurring i...

Sixty five testicular tumours in 27 years accounted for an average of 2-3 cases (specimens) per year. The estimated frequency was therefore 0.04% out of total 165,000 biopsies received during this period. Specimens were sent from surgical units of various hospitals of Karachi be sides Jinnah Postgraduate Medical Centre (JPMC). Sections prepared were stained with routine haematoxylin and eosin; ...