Periodontal disease is a widespread disease, which without proper treatment, may lead to tooth loss in adults. Because stem cells from the inflammatory microenvironment created by periodontal disease exhibit impaired regeneration potential even under favorable conditions, it is difficult to obtain satisfactory therapeutic outcomes using traditional treatments, which only focus on the control of...

BACKGROUND AIMS Recently, it has been found that effective periodontal regeneration can be induced by bone marrow mesenchymal stromal cell (BMSC) transplantation or local application of basic fibroblast growth factor (bFGF). The aim of the present study was to assess, in dogs, the efficacy of periodontal regeneration via the delivery of BMSC transfected with bFGF to repair destruction of period...

Background: Periodontal ligament stem cells (PDLSCs) are considered as a type of mesenchymal stem cell that is beneficial target for numerous clinical applications in periodontal tissue regeneration therapy. Materials and Methods: This study examined the effects of dexamethasone (Dex) on human PDLSCs in vitro. PDLSCs obtained from the roots of patient’s teeth were cultured with Dex (0....

BACKGROUND Regenerative periodontal therapy aims to predictably restore the tooth's supporting periodontal tissues and should result in formation of a new connective tissue attachment (i.e. new cementum with inserting periodontal ligament fibres) and new alveolar bone. Histologic evidence from preclinical models has demonstrated periodontal regeneration following treatment with barrier membrane...

Cell-based therapy is considered a promising approach to achieving predictable periodontal regeneration. In this study, the regenerative potential of cell sheets derived from different parts of the periodontium (gingival connective tissue, alveolar bone and periodontal ligament) were investigated in an athymic rat periodontal defect model. Periodontal ligament (PDLC), alveolar bone (ABC) and gi...

Periodontitis is a chronic inflammatory disease which leads to destruction of both the soft and hard tissues of the periodontium. Tissue engineering is a therapeutic approach in regenerative medicine that aims to induce new functional tissue regeneration via the synergistic combination of cells, biomaterials, and/or growth factors. Advances in our understanding of the biology of stem cells, inc...

Plateletrich rich fibrin (PRF) is an autologous healing biomaterial, which incorporates a matrix of autologous fibrin, leukocytes, platelets and growth factors, harvested from a simple blood sample. Various growth factors present in PRF are well-known source of cytokines, usable for clinical applications. Grade II furcation lesion is essentially a cul-de-sac with a definite horizontal component...

The native periodontium includes cementum, a functionally oriented periodontal ligament, alveolar bone and gingiva. Pathologic and/or traumatic events may lead to the loss or damage of this anatomical structure. Since the 1970s, a number of procedures have been investigated in an attempt to restore such lost tissues. Numerous clinical trials have shown positive outcomes for various reconstructi...

BACKGROUND The ideal healing technique for periodontal tissue defects would involve the functional regeneration of the alveolar bone, cementum, and periodontal ligament, with new periodontal attachment formation. In this study, gingival fibroblasts were induced and a "sandwich" tissue-engineered complex (a tissue-engineered periodontal membrane between 2 tissue-engineered mineralized membranes)...

Periodontitis is an infectious disease that causes destruction of the tooth-attachment apparatus. Untreated periodontitis results in progressive attachment loss that may eventually lead to early tooth loss. Fortunately, research has provided evidence that in most situations chronic periodontal diseases can be treated [reviewed in Ref. (29)]. There is also evidence that periodontally involved te...