Glucose-6-phosphate isomerase (phosphoglucose isomerase [PGI]) (EC 5.3.1.9) from the hyperthermophilic archaeon Pyrococcus furiosus was purified 500-fold to homogeneity. The enzyme had an apparent molecular mass of 43 kDa and was composed of a single type of subunit of 23 kDa indicating a homodimeric (alpha(2)) structure. Kinetic constants of the enzyme were determined at the optimal pH 7 and a...

Purpose This study aims to explore how individual personal growth initiative (PGI) mediates the relationship between a positive error orientation and job crafting. Furthermore, it explores moderating role of feedback from leader in this relationship. Design/methodology/approach Data was collected through survey conducted on 209 international employees multiple occupations. Findings A is indirec...

Rapid changes in climate may impose strong selective pressures on organisms. Evolutionary responses to climate change have been observed in natural populations, yet no example has been documented for a metabolic enzyme locus. Furthermore, few studies have linked physiological responses to stress with allozyme genotypic variation. We quantified changes in allele frequency between 1988 and 1996 a...

Operators of parking guidance and information (PGI) systems often have difficulty in providing the best car park availability information to drivers in periods of high demand. A new PGI configuration model based on the optimized combination method was proposed by analyzing of parking choice behavior. This article first describes a parking choice behavioral model incorporating drivers perception...

introduction: although tolerance to and dependence on opioids are characterized by excessive activity of camp pathway in some brain stem nuclei, the impact of morphine dependence on activity of camp pathway in paragigantocellularis nucleus (pgi), located in the rostral ventrolateral medulla, remains unclear. therefore, the effect of adenyl cyclase activator forskolin on spontaneous firing rate ...

Portal hypertension (PHT) is a common complication of liver cirrhosis and significantly increases morbidity and mortality. Abrogation of PHT using NSAIDs has demonstrated that prostacyclin (PGI(2)), a direct downstream metabolic product of cyclooxygenase (COX) activity, is an important mediator in the development of experimental and clinical PHT. However, the role of COX isoforms in PGI(2) bios...

Several indications exist that prostacyclin (PGI(2)) release in the cardiovascular system might be affected by cyclooxygenase (COX)-2-specific inhibitors. This could reflect an inhibition of PGI(2) synthesis in the endothelium although in these cells mainly COX-1 is expressed. Inflammation and stress induce COX-2 in smooth muscle cells which could have happened in patients with cardiac diseases...

The patient-generated index (PGI) is a more novel approach to evaluating health-related quality of life (HRQOL) that allows patients to formulate their own responses in an open-ended format in order to measure HRQOL based on each patient's own stated goals and expectations. To date the use of PGI in the setting of patients diagnosed with cancer remains relatively less common compared to other h...

One-step enzyme purification and immobilization were developed based on simple adsorption of a family 3 cellulose-binding module (CBM)-tagged protein on the external surface of high-capacity regenerated amorphous cellulose (RAC). An open reading frame (ORF) Cthe0217 encoding a putative phosphoglucose isomerase (PGI, EC 5.3.1.9) from a thermophilic bacterium Clostridium thermocellum was cloned a...

Parking Guidance and Information (PGI) signs are thought to enable a more efficient use of the available parking stock. Despite the installation of PGI systems in many cities and their operation for a number of years, there is a lack of reliable evidence of the size of the benefits that these systems can achieve. This paper describes the development of driver parking choice models (both during ...